Sec8 Antibody

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-03375
    • Availability
      Product Discontinued

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Sec8 Antibody Summary

Immunogen
A synthetic peptide from the n-terminal of Exocyst complex component 4 (EXOC4, SEC8, SEC8 L1) conjugated to an immunogenic carrier protein was used as the immunogen. The immunogen is homologous in mouse and rat.
Specificity
EXOC4.
Clonality
Polyclonal
Host
Rabbit
Gene
EXOC4
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Positive Control
Sec8 Lysate (NBL1-10379)

Reactivity Notes

Mouse, Rat and Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Concentration
LYOPH
Purity
Unpurified
Reconstitution Instructions
Reconstitute with 0.1 ml sterilized water to desired concentration.

Alternate Names for Sec8 Antibody

  • exocyst complex component 4
  • KIAA1699SEC8L1
  • MGC27170
  • REC8
  • SEC8Exocyst complex component Sec8
  • SEC8-like 1 (S. cerevisiae)
  • SEC8-like 1
  • Sec8p

Background

Sec8 is a 110 kDa homolog of yeast Sec8, a protein that is essential for protein transport between the Golgi apparatus and the plasma membrane. In yeast, Sec8 is part of a 19.5S complex that includes Sec6 and Sec15, that also associate with the ras-like GTP binding protein Sec4. An analogous 17S complex in rat brain was found to contain Sec6 and Sec8, and eight novel proteins with a combined molecular weight of 743kDa. COS cell cotransecfection analysis revealed that rSec8 colocalized with GTP binding protein Rab3a and syntaxin 1a. Membrane-associated Sec6/8 complex co-immunoprecipitated with syntaxin, provided further evidence that this complex is involved in synaptic vesicle docking and fusion at the presynaptic terminal.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Sec8 Products

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Bioinformatics

Gene Symbol EXOC4
Entrez
Uniprot