SCARA5 Antibody

Images

 
Western Blot: SCARA5 Antibody [NBP1-77050] - Human liver tissue lysate with SCARA5 antibody at 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: SCARA5 Antibody [NBP1-77050] - Immunofluorescence of SCARA5 in HepG2 cells with SCARA5 antibody at 20 ug/mL.
Immunocytochemistry/ Immunofluorescence: SCARA5 Antibody [NBP1-77050] - HepG2 cells with SCARA5 antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, BvSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

SCARA5 Antibody Summary

Immunogen
Antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SCARA5. The immunogen is located within amino acids 350 - 400 of SCARA5. Amino Acid Squence: KGDRGPKGEKGEKGDRA
Specificity
At least four isoforms of SCARA5 are known to exist; this antibody will detect all four isoforms.
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SCARA5
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 2.5-20 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 29461673).
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
SCARA5 Peptide (NBP1-77050PEP)
Publications
Read Publication using NBP1-77050.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Chicken (86%), Rat (80%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for SCARA5 Antibody

  • FLJ23907
  • MGC45780
  • SCARA5
  • scavenger receptor class A, member 5 (putative)
  • Tesr
  • testis expressed scavenger receptor
  • UNQ2938/PRO28700

Background

Scavenger receptor class A member 5 (SCARA5), also known as TESR, is part of the scavenger receptor superfamily, which is composed of many members with diverse structures, expression patterns, and functions. SCARA5 is a cell-surface receptor that contains that collagen-like domain and N-terminal scavenger receptor cysteine rich domain typical of the class A scavenger receptor subfamily. Unlike other class A receptors, SCARA5 does not endocytose acetylated or oxidized low density lipoprotein. Instead, SCARA5 binds serum ferritin and mediates its endocytosis from the cell surface, indicating SCARA5 may play a role in non-transferrin iron delivery. At least four isoforms of SCARB1 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SCARA5 Antibody (NBP1-77050)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SCARA5 Antibody (NBP1-77050). (Showing 1 - 1 of 1 FAQ).

  1. We are trying to detect chicken SCARA5 protein by western blot using NBP1-77050 antibody (Antibody was raised against a 17 amino acid peptide near the carboxy terminus of human SCARA5). I am wondering whether the antibody can detect the chicken SCARA5 protein based on homology between human and chicken. Can I get AA sequences of the antigenic peptide?
    • I have now heard back from the lab in response to your enquiry. Although they cannot disclose the amino acid sequence of the antigenic peptide, they have analyzed how the immunogen compares to the equivalent sequence in chicken, and there is 70% homology. Unfortunately, based on this percentage and distribution of homologous amino acids, it is difficult to predict whether the antibody will be able to detect the chicken SCARA5 protein.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SCARA5
Uniprot