Scaffold attachment factor B2 Antibody (5A11)

Images

 
Immunohistochemistry-Paraffin: Scaffold attachment factor B2 Antibody (5A11) [NBP1-69834] - Analysis of anti-SAFB antibody with human prostate at concentration 5 ug/ml.
Immunohistochemistry-Paraffin: Scaffold attachment factor B2 Antibody (5A11) [NBP1-69834] - Analysis of anti-SAFB antibody with human uterus at concentration 5 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC-P, IF
Clone
5A11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Scaffold attachment factor B2 Antibody (5A11) Summary

Immunogen
SAFB (NP_002958, 111 a.a. - 201 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SAFB2
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunofluorescence 10 ug/ml

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein A purified

Alternate Names for Scaffold attachment factor B2 Antibody (5A11)

  • KIAA0138SAF-B2
  • scaffold attachment factor B2

Background

Activity of the estrogen receptor (ER) is regulated through interaction with coactivators and corepressors. These proteins are present in large complexes, suggesting functional interactions among various cofactors. The scaffold attachment factor SAFB2, a component of high molecular weight nuclear protein complexes which contains a core set of protein interaction partners, functions as an estrogen receptor corepressor. It directly interacts with ER alpha repressing transcription via repression domains. Its over expression results in inhibition of proliferation. SAFB2 is found in the cytoplasm, as well as the nucleus. Consistent with its cytoplasmic localization, there is also an interaction between SAFB2 and vinexin, a protein involved in linking signaling to the cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SAFB2