SART1 Antibody

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Western Blot: SART1 Antibody [NB100-68232] - Detection of Human SART1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from (1 mg for IP, 20% of IP loaded) HeLa cells. Antibodies: Affinity purified ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-68232
    • Availability
      Product Discontinued

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SART1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 675 and 725 of human squamous cell carcinoma antigen recognized by T cells using the numbering given in entry NP_005137.1 (GeneID 9092).
Clonality
Polyclonal
Host
Rabbit
Gene
SART1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunoprecipitation 1:10-1:500

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SART1 Antibody

  • Allergen Hom s 1
  • Ara1
  • HAF
  • HOMS1
  • hSART-1
  • hSnu66
  • hypoxia-associated factor
  • IgE Autoantigen
  • MGC2038
  • SART1
  • SART-1
  • SART1(259) protein
  • SART1(800) protein
  • SART1259
  • small nuclear ribonucleoprotein 110kDa (U4/U6.U5)
  • SNRNP110
  • SNU66 homolog
  • Snu66
  • squamous cell carcinoma antigen recognised by T cells
  • Squamous cell carcinoma antigen recognized by T cells 1
  • squamous cell carcinoma antigen recognized by T cells
  • U4/U6.U5 tri-snRNP-associated 110 kDa protein
  • U4/U6.U5 tri-snRNP-associated protein 1

Background

This gene encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frame shifting during post transcriptional regulation; its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SART1
Entrez
Uniprot