SARS Nucleocapsid Protein Antibody

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Western Blot: SARS Nucleocapsid Protein Antibody [NBP1-78110] - Shows detection of a 34-kDa band corresponding to the protein. Approx. 100 ug of protein was loaded for SDS-PAGE and transferred onto nitrocellulose. The ...read more

Product Details

Summary
Reactivity ViSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SARS Nucleocapsid Protein Antibody Summary

Immunogen
This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant protein corresponding to full length SARS Coronavirus 3CL Protease.prepared the 3 epared the 3CL Protease as follows: SUMO-3CL protease fusion was expressed in E. coli in LB medium and purified by Ni-NTA resin affinity chromatography. After the fusion was cleaved by the SUMO Protease, the SUMO tag and protease were subtracted from the 3CL protease using MAC and the 3CL protease was finally purified using Anion Exchange Chromatography with the Macro-Prep High Q resin and size exclusion chromatography.
Specificity
This protein A purified antibody is directed against SARS Coronavirus 3CL Protease. A BLAST analysis was used to suggest reactivity with related Coronavirus proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
N
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • ELISA 1:10000-1:50000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
This protein A purified antibody has been tested for use in ELISA and by western blot. Expect a band approximately 34 kDa in size corresponding - SARS 3CL Protease by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

Reactive in virus

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Protein A purified

Alternate Names for SARS Nucleocapsid Protein Antibody

  • N protein
  • N structural protein
  • N
  • NC
  • Nucleocapsid protein
  • SARS coronavirus N protein
  • SARS coronavirus nucleocapsid protein
  • SARS CoV N protein
  • SARS CoV nucleocapsid protein
  • SARS CoV
  • SARS N protein
  • SARSCoV N protein
  • SARSCoV nucleocapsid protein
  • SARSCoV
  • Severe acute respiratory syndrome

Background

Generally, viruses have proteases to process their proteins into active form. Because of its pivotal role in the viral life cycle, proteases are primary targets for the development of antiviral agents. 3CL protease, a viral cysteine proteinase, plays an important role in co-translational proteolytic processing of Coronavirus polyproteins. The 3CL protease cleaves as much as 11 sites in the replicase polyproteins and also releases the key replicative functions of polymerase and helicase. 3CL protease is the only Coronavirus protein for which structural information is available. 3CL protease comprises three domains, the substrate-binding site is expected to be located between domains I and II, and domain III is a globular cluster comprising five helices. 3CL protease is a homodimer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol N
Uniprot