Simple Western: SARS Nucleocapsid Protein Antibody [NB100-56683] - Simple Western lane view shows recombinant SARS-CoV-2 Nucleocapsid Protein (Catalog # 10474-CV), loaded at 20 ng/mL. A specific band was detected for ...read more
Simple Western: SARS Nucleocapsid Protein Antibody [NB100-56683] - Simple Western lane view shows lysates of SARS-CoV-2 (1:50), MERS (1:100), OC43 (1:100), and 229E (1:100). A specific band was detected for SARS-CoV-2 ...read more
Western Blot: SARS Nucleocapsid Protein Antibody [NB100-56683] - Analysis of SARS Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate using this antibody at a 1:2000 dilution.
The antibody was developed by immunizing rabbits with a synthetic peptide corresponding to amino acids 354-370 (LNKHIDAYKTFPPTEPK-C) from the N (SARS Nucleocapsid) for the Human SARS coronavirus (Genbank accession no. NP_828858.1)
The was tested on a human cell line transfected with full-length SARS Nucleocapsid cDNA with a predicted molecular weight of 46 kDa.
SARS-CoV-2 (100%). Backed by our 100% Guarantee.
Protein G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Expected species cross reactivity based on immunogen sequence homology: COVID-19 (100%)
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein G purified
Alternate Names for SARS Nucleocapsid Protein Antibody
COVID-19 nucleocapsid protein
N structural protein
SARS coronavirus N protein
SARS coronavirus nucleocapsid protein
SARS CoV N protein
SARS CoV nucleocapsid protein
SARS N protein
SARSCoV N protein
SARSCoV nucleocapsid protein
SARSCOV2 N protein
SARS-COV-2 nucleocapsid protein
Severe acute respiratory syndrome
It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for SARS Nucleocapsid Protein Antibody (NB100-56683)(5)
We have publications tested in 2 applications: ICC/IF, WB.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.