Sars Membrane Protein Antibody

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Immunocytochemistry/ Immunofluorescence: Sars Membrane Protein Antibody [NBP2-21628] - Epithelial cells fixed and stained using PFA 20 hours post infection. Staining: SARS CoV Membrane Far Red, Phalloidin F-actin, Actin ...read more

Product Details

Summary
Reactivity VSpecies Glossary
Applications WB, EM, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Sars Membrane Protein Antibody Summary

Immunogen
This Sars Membrane Protein Antibody was prepared by repeated immunizations with a BSA-coupled synthetic peptide corresponding to the C-terminus (amino acid residues 204-221, C-KLNTDHAGSNDNIALLVQ) of the Human SARS Coronavirus M (Membrane protein) (GenBank: AAP13444).
Clonality
Polyclonal
Host
Rabbit
Purity
Whole Antiserum
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Electron Microscopy 1:200
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunoprecipitation 1:60
Application Notes
Western Blot: Expect a band of approximately 25 kDa in size corresponding to SARS-CoV membrane protein by western blotting in the appropriate cell lysate or extract.

ICC/IF: Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37 degrees C. Infection occurred in PBS/DEAE/2%FCS followed by exchange to EMEM/25mMHEPES/2%FCS. Cells were fixed with PBS/3%PFA. After washing fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min.

Reactivity Notes

Expected species cross reactivity based on immunogen sequence homology: COVID-19 (83%)

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
0.01% Sodium Azide
Purity
Whole Antiserum

Notes

Store vial at -20 C prior to opening. Aliquot contents and freeze at -20 C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for Sars Membrane Protein Antibody

  • E1 glycoprotein
  • Matrix glycoprotein
  • Membrane glycoprotein
  • SARS coronavirus M protein
  • SARS coronavirus Membrane protein
  • SARS CoV M glycoprotein
  • SARS CoV Matrix protein
  • SARS CoV
  • Sars M
  • Sars Matrix Protein
  • SARS-CoV M protein
  • SARS-CoV Membrane protein
  • SARSCoV
  • Severe acute respiratory syndrome

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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