Sars Membrane Protein Antibody


Immunocytochemistry/ Immunofluorescence: Sars Membrane Protein Antibody [NBP2-21628] - Epithelial cells fixed and stained using PFA 20 hours post infection. Staining: SARS CoV Membrane Far Red, Phalloidin F-actin, Actin more

Product Details

Reactivity VSpecies Glossary
Applications WB, EM, ICC/IF, IP

Order Details

Sars Membrane Protein Antibody Summary

This Sars Membrane Protein Antibody was prepared by repeated immunizations with a BSA-coupled synthetic peptide corresponding to the C-terminus (amino acid residues 204-221, C-KLNTDHAGSNDNIALLVQ) of the Human SARS Coronavirus M (Membrane protein) (GenBank: AAP13444).
Whole Antiserum
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  • Western Blot 1:1000
  • Electron Microscopy 1:200
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunoprecipitation 1:60
Application Notes
Western Blot: Expect a band of approximately 25 kDa in size corresponding to SARS-CoV membrane protein by western blotting in the appropriate cell lysate or extract.

ICC/IF: Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37 degrees C. Infection occurred in PBS/DEAE/2%FCS followed by exchange to EMEM/25mMHEPES/2%FCS. Cells were fixed with PBS/3%PFA. After washing fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min.

Reactivity Notes

Expected species cross reactivity based on immunogen sequence homology: COVID-19 (83%)

Packaging, Storage & Formulations

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
No buffer
0.01% Sodium Azide
Whole Antiserum


Store vial at -20 C prior to opening. Aliquot contents and freeze at -20 C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for Sars Membrane Protein Antibody

  • E1 glycoprotein
  • Matrix glycoprotein
  • Membrane glycoprotein
  • SARS coronavirus M protein
  • SARS coronavirus Membrane protein
  • SARS CoV M glycoprotein
  • SARS CoV Matrix protein
  • SARS CoV
  • Sars M
  • Sars Matrix Protein
  • SARS-CoV M protein
  • SARS-CoV Membrane protein
  • Severe acute respiratory syndrome


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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