SARS-CoV nsp3 Antibody

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Immunocytochemistry/ Immunofluorescence: SARS-CoV nsp3 Antibody [NBP1-78109] - 6-h post infection Vero-E6 cells. For detection Cy3 conjugated Goat-anti-Rabbit IgG MX was used.

Product Details

Summary
Reactivity VSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SARS-CoV nsp3 Antibody Summary

Immunogen
This SARS-CoV nsp3 Antibody was produced by repeated immunizations with 3 E.coli derived proteins representing different parts of the SARS-CoV Nsp3 protein: aa 834-943 of polyprotein 1a (ADRP domain), aa 1208-1557 of polyprotein 1a (SARS-Unique Domain) and aa 1558-2040 of polyprotein 1a (Papain-like proteinase domain).
Specificity
This antibody is directed against SARS-Coronavirus nsp3 protein. Cross reactivity with homologues from other sources has not been determined.
Clonality
Polyclonal
Host
Rabbit
Gene
orf1ab
Purity
Whole Antiserum
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunoprecipitation 1:60
Application Notes
Western Blot: Expect a band of approximately 211 kDa in size corresponding to SARS-CoV nsp3 by western blotting in the appropriate cell lysate or extract.

ICC/IF: Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37C. Infection occurred in PBS/DEAE/2%FCS followed by exchange to EMEM/25mMHEPES/2%FCS. Cells were fixed with PBS/3%PFA. After washing fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min.

Reactivity Notes

Cross reactivity with homologues from other sources has not been determined.

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
None
Preservative
0.01% Sodium Azide
Purity
Whole Antiserum

Notes

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for SARS-CoV nsp3 Antibody

  • orf1ab polyprotein (pp1ab)

Background

The nonstructural protein 3 (nsp3) is one of the SARS-Coronavirus replicase cleaving products, encoded by ORF1a. Nsp3 is thought to be part of the viral replication complex, which is associated with intracellular membranes. Nsp3 contains a papain-like cysteine proteinase and ADP-ribose-1'-monophosphatase activities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SARS-CoV nsp3 Antibody (NBP1-78109) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional SARS-CoV nsp3 Products

Array NBP1-78109

Bioinformatics Tool for SARS-CoV nsp3 Antibody (NBP1-78109)

Discover related pathways, diseases and genes to SARS-CoV nsp3 Antibody (NBP1-78109). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Calculators

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Bioinformatics

Gene Symbol orf1ab
Uniprot