Sarcosine oxidase subunit A Antibody

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Western Blot: Pipecolic acid oxidase Antibody [NB600-649] - Analysis using HRP conjugate of NB600-649. Detection of Sarcosine Oxidase. Samples of 1 and 0.25 ug of purified Sarcosine Oxidase per lane were run by SDS-PAGE ...read more
Western Blot: Pipecolic acid oxidase Antibody [NB600-649] - Detection of Sarcosine Oxidase. Samples of 1 and 0.25 ug of purified Sarcosine Oxidase per lane were run by SDS-PAGE under reducing and non-reducing conditions ...read more
Western Blot: Pipecolic acid oxidase Antibody [NB600-649] - Analysis using HRP conjugate of NB600-649. Lane 1: Sarcosine Oxidase (Microbial). Load: 50 ng per lane. Primary antibody: Goat anti-Sarcosine Oxidase ...read more

Product Details

Summary
Reactivity BaSpecies Glossary
Applications WB, ELISA, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

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Catalog# & Conjugate Size Price

Sarcosine oxidase subunit A Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Sarcosine Oxidase (Microbial)
Specificity
Assay by immunoelectrophoresis resulted in a single precipition arc against anti-Goat Serum as well as purified and partially purified Sarcosine Oxidase (Microbial).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Ion exchange chromatography
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:5000
  • ELISA 1:5000 - 1:20000
  • Immunoprecipitation 1:100
Application Notes
This product has been assayed against 1.0 ug of Sarcosine Oxidase [Microbial] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:3,000 - 1:12,000 of the reconstitution concentration is suggested for this product.

Reactivity Notes

Cross reactivity against Sarcosine Oxidase from other sources is unknown.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Ion exchange chromatography

Background

Sarcosine Oxidase catalyzes the oxidative demethylation of sarcosine. It can also oxidize other secondary amino acids such as N methyl L alanine, L pipecolic acid and L proline.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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