SAFB Antibody (6F7)

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Immunohistochemistry-Paraffin: Scaffold attachment factor B2 Antibody (6F7) [NBP1-50193] - Analysis of anti-SAFB antibody with human brain, cortex at concentration 5 ug/ml.

Product Details

Summary
Reactivity Hu, Mk, PmSpecies Glossary
Applications WB, IHC-P, IP, IF
Clone
6F7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

SAFB Antibody (6F7) Summary

Immunogen
Synthetic peptide from human SAFB.
Specificity
A synthetic peptide corresponding to amino acids 345-357 (PEARDSKEDGRKF) of the human HET protein.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SAFB
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Immunofluorescence 1:10 - 1:500

Reactivity Notes

Gorilla, Orangutan, Gibbon, Marmoset.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS. Sourced in TCS.
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for SAFB Antibody (6F7)

  • glutathione S-transferase fusion protein
  • HAP
  • heat-shock protein (HSP27) estrogen response element and TATA box-bindingprotein
  • HETDKFZp779C1727
  • Hsp27 ERE-TATA binding protein
  • HSP27 ERE-TATA-binding protein
  • HSP27 estrogen response element-TATA box-binding protein
  • SAF-B
  • SAF-B1
  • SAFB1SAB-B1
  • scaffold attachment factor B
  • scaffold attachment factor B1

Background

This gene encodes a DNA-binding protein that has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. This encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of the heat shock protein 27 transcription and also can act as an estrogen receptor corepressor. This gene is a candidate gene for breast tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SAFB