SAE2 Antibody

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Western Blot: SAE2 Antibody [NBP1-49997] - Detection of Human and Mouse SAE2 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-49997
    • Availability
      Product Discontinued

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SAE2 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 525 and 575 of human SUMO1 Activating Enzyme Subunit 2 using the numbering given in entry NP_005490.1 (GeneID 10054).
Clonality
Polyclonal
Host
Rabbit
Gene
UBA2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 2-5 mcg/mg lysate

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SAE2 Antibody

  • Anthracycline-associated resistance ARX
  • ARX
  • EC 6.3.2
  • EC 6.3.2.-
  • FLJ13058
  • HRIHFB2115
  • SAE2SUMO-1 activating enzyme subunit 2
  • SUMO1 activating enzyme subunit 2
  • SUMO-activating enzyme subunit 2
  • UBA2, ubiquitin-activating enzyme E1 homolog
  • Ubiquitin-like 1-activating enzyme E1B
  • ubiquitin-like modifier activating enzyme 2
  • UBLE1B

Background

The human UBiquitin Activating domain 2 (UBA2) gene is located on chromosome 19q12. Human UBA2 could form a beta mercaptoethanol-sensitive conjugate with members of the sentrin family, but not with ubiquitin of NEDD8 in the presence of AOS1. The recombinant proteins, Sua1p and hUba2, form a complex. In this complex, hUba2 binds SUMO-1, which gives the complex the activity of the SUMO-1 activating enzyme. Therefore, UBA2 has been found to be a component of the sentrin-activating enzyme complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol UBA2