Recombinant Human RSK3 Protein

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SDS-Page: Recombinant RSK3 Protein [NBP1-99503]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity
Concentration
0.1 mg/ml

Order Details

Recombinant Human RSK3 Protein Summary

Description
Recombinant protein for Human RSK3

Source:Baculovirus (Sf9 insect cells)
Amino Acid Sequence:(NM_021135)

Source
Sf 9 (baculovirus)
Protein/Peptide Type
Recombinant Protein
Gene
RPS6KA2
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Application Notes
Unit Definition: One unit is defined as the amount of enzyme that will hydrolyze one pmol Suc-LLVY-AMC per minute at 25 C.
Specific activity: The specific activity of RSK3 was determined to be 131 nmol /min/mg as per activity assay protocol.
Theoretical MW
112 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol
Concentration
0.1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human RSK3 Protein

  • EC 2.7.11
  • EC 2.7.11.1
  • HU-2
  • MAP kinase-activated protein kinase 1c
  • MAPK-activated protein kinase 1c
  • MAPKAP kinase 1c
  • MAPKAPK1C
  • MAPKAPK-1c
  • p90-RSK 2
  • p90RSK2
  • p90-RSK3
  • ribosomal protein S6 kinase alpha 2
  • ribosomal protein S6 kinase alpha-2
  • ribosomal protein S6 kinase, 90kD, polypeptide 2
  • ribosomal protein S6 kinase, 90kDa, polypeptide 2
  • Ribosomal S6 kinase 3,90 kDa ribosomal protein S6 kinase 2
  • RPS6KA2
  • RSK
  • RSK3
  • RSK-3
  • RSK3pp90RSK3
  • S6K-alpha
  • S6K-alpha2
  • S6K-alpha-2

Background

RSK3 is a member of the RSK (ribosomal S6 kinase) family that encodes a 733-amino-acid protein with a unique N-terminal region containing a putative nuclear localization signal (1). RSK3 mRNA is widely expressed and is activated by growth factors, serum and phorbol ester. Upon stimulation, RSK3 translocates to the cell nucleus and phosphorylates nuclear proteins. RSK3 can bind to ERK1/2 and this association increases the duration of RSK3 activation (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol RPS6KA2
Entrez