RPTOR Antibody

Images

 
Western Blot: RPTOR Antibody [NBP1-47166] - Western blot analysis (left) of RPTOR in L1210 cell lysate wanti-RPTOR (CT) at (A) 2 and (B) 4 ug/ml.
Immunocytochemistry/ Immunofluorescence: RPTOR Antibody [NBP1-47166] - Immunocytochemistry (right) of RPTOR in L1210 cells with anti-RPTOR (CT) at 10 ug/ml.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-47166
    • Availability
      Product Discontinued

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RPTOR Antibody Summary

Immunogen
Rabbit polyclonal anti-Raptor (CT) was raised against a 16 amino acid peptide from near the carboxy-terminus of mouse Raptor (Genbank accession No. Q8K4Q0).
Clonality
Polyclonal
Host
Rabbit
Gene
RPTOR
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000

Reactivity Notes

mouse

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Purity
IgG purified

Alternate Names for RPTOR Antibody

  • KIAA1303
  • KIAA1303p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats
  • KOG1
  • Mip1
  • p150 target of rapamycin (TOR)-scaffold protein
  • Raptor
  • regulatory associated protein of MTOR, complex 1
  • regulatory-associated protein of mTOR
  • RPTOR

Background

mTOR controls cell growth, in part by regulating p70 S6 kinase alpha (p70alpha) and eukaryotic initiation factor 4E binding protein 1 (4EBP1). Raptor is a 150 kDa mTOR binding protein that also binds 4EBP1 and p70alpha. The binding of Raptor to mTOR is necessary for the mTOR-catalyzed phosphorylation of 4EBP1 in vitro, and it strongly enhances the mTOR kinase activity toward p70alpha. Rapamycin or amino acid withdrawal increases, whereas insulin strongly inhibits, the recovery of 4EBP1 and raptor on 7-methyl-GTP Sepharose. Partial inhibition of raptor expression by RNA interference (RNAi) reduces mTOR-catalyzed 4EBP1 phosphorylation in vitro. RNAi of C. elegans raptor yields an array of phenotypes that closely resemble those produced by inactivation of Ce-TOR. Thus, raptor is an essential scaffold for the mTOR-catalyzed phosphorylation of 4EBP1 and mediates TOR action in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RPTOR
Entrez