RPTOR Antibody

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Biological Strategies: Western Blot: RPTOR Antibody [NB100-331] - Detection of Human and Mouse Raptor by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using ...read more
Western Blot: RPTOR Antibody [NB100-331] - Whole cell lysate (20 ug) from rat L6, mouse C2C12 and human HEK293 cells (lanes 1, 2 and 3, respectively). Antibody used at 0.2 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

RPTOR Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 975 and 1025 of human Regulatory associated protein of mTOR using the numbering given in entry AAM09075.1 (GeneID 57521)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPTOR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
Application Notes
Immunoprecipitation - Not recommended, use NB 100-766 for IP.
Publications
Read Publications using
NB100-331 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RPTOR Antibody

  • KIAA1303
  • KIAA1303p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats
  • KOG1
  • Mip1
  • p150 target of rapamycin (TOR)-scaffold protein
  • Raptor
  • regulatory associated protein of MTOR, complex 1
  • regulatory-associated protein of mTOR
  • RPTOR

Background

Raptor (regulatory associated protein of mTOR) is a 150 kDa mTOR (Target of Rapamycin) binding protein. It plays a positive role in nutrient-stimulated signaling and cell size maintenance.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RPTOR Antibody (NB100-331)(6)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RPTOR
Entrez
Uniprot