![SDS-Page: Recombinant Plant rPhl p 12 Strep (N-Term) Protein [NBP3-07895] - Recombinant allergen rPhl p 12 purity verification. 5 ug of rPhl p 12 with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.](http://images.novusbio.com/fullsize/Recombinant-Plant-rPhl-p-12-Strep-N-Term-Protein-SDS-Page-NBP3-07895-img0001.jpg "SDS-Page: Recombinant Plant rPhl p 12 Strep (N-Term) Protein [NBP3-07895] - Recombinant allergen rPhl p 12 purity verification. 5 ug of rPhl p 12 with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.")
| Reactivity | PlSpecies Glossary |
| Applications | ELISA |
| Format | Carrier-Free |
| Concentration | LYOPH |
| Description | A recombinant protein with an N-terminal Strep-tag corresponding to the Phleum pratense protein rPhl p 12. Source: E.coli 143 amino acid sequence. |
| Preparation Method |
The protein was purified by ionex and affinity chromatography, using Strep-tag. Endotoxin was removed using a specific endotrap carrier. Product was lyophilized after purification. |
| Source | E. coli |
| Protein/Peptide Type | Recombinant Protein |
| Purity | >95%, by HPLC |
| Dilutions |
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| Theoretical MW | 15.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 100 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 8.0 |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | >95%, by HPLC |
| Reconstitution Instructions | Reconstitute with sterile deionized water with or without azide. |
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