RPB2 Antibody

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Immunoprecipitation: RPB2 Antibody [NB100-333] - Samples: Whole cell lysate (50 ug for WB; 10 cm plate for IP) from 293T cells. Antibodies: Affinity purified rabbit anti-Rpb2 (BL940) used at the indicated concentration ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

RPB2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 75 and 125 of human RNA Polymerase 2 Polypeptide B using the numbering given in entry NP_000929.1 (GeneID 5431).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLR2B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Uses: NB 100-333 may be used for Western Blot and immunoprecipitation. Suggested working dilutions: * Western Blot - 1:1,000-1:5,000 (ECL) Immunohisto/cytochemistry - Not Determined Immunoprecipitation - 2 to 5 ug/mg lysate *The investigator should determine the optimal working dilution for a specific application.
Publications
Read Publication using
NB100-333 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RPB2 Antibody

  • DNA-directed RNA polymerase II subunit RPB2
  • EC 2.7.7
  • EC 2.7.7.6
  • hRPB140
  • hsRPB2
  • POL2RB
  • polymerase (RNA) II (DNA directed) polypeptide B (140kD)
  • polymerase (RNA) II (DNA directed) polypeptide B, 140kDa
  • RNA polymerase II subunit 2
  • RNA polymerase II subunit B2
  • RNA polymerase II subunit2, RNA polymerase II second largest subunit

Background

It is suggested that Rpb2, a 140 kDa protein, plays a role is enhancing transcription from prostate specific antigen genes. These genes may be linked to prostate cancer progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IP

Publications for RPB2 Antibody (NB100-333)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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WB
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol POLR2B
Entrez
Uniprot