RPA2 Antibody (1H10)

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Western Blot: RPA2 Antibody (1H10) [NBP2-02184] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-RPA2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: ...read more
Immunocytochemistry/ Immunofluorescence: RPA2 Antibody (1H10) [NBP2-02184] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RPA2.
Immunohistochemistry-Paraffin: RPA2 Antibody (1H10) [NBP2-02184] - Staining of paraffin-embedded Human prostate tissue using anti-RPA2 mouse monoclonal antibody.
Flow Cytometry: RPA2 Antibody (1H10) [NBP2-02184] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RPA2 antibody, and then analyzed ...read more
Western Blot: RPA2 Antibody (1H10) [NBP2-02184] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunohistochemistry-Paraffin: RPA2 Antibody (1H10) [NBP2-02184] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RPA2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RPA2 Antibody (1H10) [NBP2-02184] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RPA2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RPA2 Antibody (1H10) [NBP2-02184] - Staining of paraffin-embedded Human breast tissue using anti-RPA2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RPA2 Antibody (1H10) [NBP2-02184] - Staining of paraffin-embedded Human colon tissue using anti-RPA2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RPA2 Antibody (1H10) [NBP2-02184] - Staining of paraffin-embedded Human Kidney tissue using anti-RPA2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RPA2 Antibody (1H10) [NBP2-02184] - Staining of paraffin-embedded Human Ovary tissue using anti-RPA2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RPA2 Antibody (1H10) [NBP2-02184] - Staining of paraffin-embedded Human pancreas tissue using anti-RPA2 mouse monoclonal antibody.
Flow Cytometry: RPA2 Antibody (1H10) [NBP2-02184] - Analysis of Hela cells, using anti-RPA2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: RPA2 Antibody (1H10) [NBP2-02184] - Analysis of Jurkat cells, using anti-RPA2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-02184
    • Availability
      Product Discontinued

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RPA2 Antibody (1H10) Summary

Immunogen
Full length human recombinant protein of human RPA2 (NP_002937) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RPA2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation 2ug/500ul

Reactivity Notes

Human, Dog, Rat, Monkey

Alternate Names for RPA2 Antibody (1H10)

  • REPA2
  • Replication factor A protein 2
  • replication protein A 32 kDa subunit
  • Replication protein A 34 kDa subunit
  • replication protein A2 (32kD)
  • replication protein A2, 32kDa
  • RF-A protein 2
  • RP-A p32
  • RP-A p34
  • RPA2
  • RPA32
  • RPA34

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Blogs on RPA2.

Transportin 1 and heterogeneous nuclear ribonucleoprotein D (hnRNPD)
Transportin 1, also known as Karyopherin- beta 2 or Importin- beta 2, is part of the beta -karyopherins family, which consists of importins and exportins responsible for the active transport of proteins between the nucleus and cytoplasm.  Transportin 1 is co...  Read full blog post.

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Bioinformatics

Gene Symbol RPA2
Entrez
Uniprot