RP105/CD180 Antibody (MHR73-11)

Images

 
Flow Cytometry: RP105/CD180 Antibody (MHR73-11) [NBP1-49025] - Used for binding Daudi cells in flow cytometry. This image was submitted via customer review.
Flow Cytometry: RP105/CD180 Antibody (MHR73-11) [NBP1-49025] - Human peripheral blood lymphocytes stained with purified MHR73-11, followed by anti-mouse IgG FITC

Product Details

Summary
Product Discontinued
View other related RP105/CD180 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-49025
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

RP105/CD180 Antibody (MHR73-11) Summary

Immunogen
This antibody was generated by immunizing host with Human RP105 complexed with mouse MD-1
Localization
B cells, monocytes/macrophages, and dendritic cells
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD180
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
For immunofluorescent staining, the suggested use of this reagent is 2.0 ug per million cells in 100 l volume or 100 l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-49025 in the following applications:

Reactivity Notes

Cross-Reactivity Baboon, Cynomolgus, Rhesus

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for RP105/CD180 Antibody (MHR73-11)

  • CD180 antigenMGC126233
  • CD180 molecule
  • CD180
  • Ly64
  • LY64MGC126234
  • Ly78
  • Lymphocyte antigen 64
  • lymphocyte antigen-64, radioprotective, 105kDa
  • Radioprotective 105 kDa protein
  • RP105
  • RP105lymphocyte antigen 64 (mouse) homolog, radioprotective, 105kD

Background

CD180, also known as RP105, is a 105 kD member of the toll-like receptor (TLR) family and is expressed on most B cells, monocytes/macrophages and dendritic cells. The TLR family's structural characteristics are critically involved in protein-protein signaling interactions leading to the activation of the innate immune system. Additionally, MD-1, a secretory protein, associates with CD180 (RP105) at the cell surface and this complex, in conjunction with TLR4, recognizes bacterial LPS. The human CD180 (RP105) gene maps to 5q12. The MHR73-11 antibody is able to activate B cells and to protect against irradiation- or dexamethasone-induced apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
NB100-56566
Species: Hu, Mu, Rt
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, CyTOF-ready, ELISA(Cap), Flow-CS, Flow-IC
NBP1-84550
Species: Hu
Applications: WB, IHC, IHC-P
NB100-56700
Species: Hu, Mu
Applications: WB, IHC-P
NB100-56655
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NB100-56722
Species: Hu, Ca
Applications: WB, B/N, Flow, ICC/IF, IHC-P, IP, Flow-CS
NBP2-66200
Species: Hu
NBP2-25208
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, KO
NB100-56104
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-Fr, IHC-P, IP
NBP2-29328
Species: Hu, Mu, Rt, Xp, Ye, Ze
Applications: ELISA
MAB37861
Species: Hu
Applications: WB, Simple Western, ICC
NBP2-25196
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, In vitro, CyTOF-ready
MAB140
Species: Hu
Applications: Flow, IHC, CyTOF-ready, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
NBP2-24729
Species: Hu, Mu, Rt, Ca, Eq, Mk, Pm
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, In vitro, CyTOF-ready, Flow-IC
NBP1-49025
Species: Hu, Pm
Applications: Flow, IHC, IHC-Fr, IP

Publications for RP105/CD180 Antibody (NBP1-49025) (0)

There are no publications for RP105/CD180 Antibody (NBP1-49025).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for RP105/CD180 Antibody (NBP1-49025) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-49025:
Filter by Applications
Flow
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Flow Cytometry RP105/CD180 NBP1-49025
Enlarge
reviewed by:
Anonymous
Flow Human 02/13/2018
View

Summary

ApplicationFlow Cytometry
Sample TestedDaudi cells, JJN3 cells
SpeciesHuman
LotB189680

Comments

CommentsUsed this for binding Daudi cells in flow cytometry. Highly recommended works very well

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for RP105/CD180 Antibody (NBP1-49025) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional RP105/CD180 Products

Bioinformatics Tool for RP105/CD180 Antibody (NBP1-49025)

Discover related pathways, diseases and genes to RP105/CD180 Antibody (NBP1-49025). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for RP105/CD180 Antibody (NBP1-49025)

Discover more about diseases related to RP105/CD180 Antibody (NBP1-49025).
 

Pathways for RP105/CD180 Antibody (NBP1-49025)

View related products by pathway.

PTMs for RP105/CD180 Antibody (NBP1-49025)

Learn more about PTMs related to RP105/CD180 Antibody (NBP1-49025).
 

Research Areas for RP105/CD180 Antibody (NBP1-49025)

Find related products by research area.

Blogs on RP105/CD180

There are no specific blogs for RP105/CD180, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
02/13/2018
Application: Flow
Species: Human

Bioinformatics

Gene Symbol CD180
Entrez