RP105/CD180 Antibody (MHR73-11)

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Flow Cytometry: RP105/CD180 Antibody (MHR73-11) [NBP1-49025] - Human peripheral blood lymphocytes stained with purified MHR73-11, followed by anti-mouse IgG FITC

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications Flow, IHC, IHC-Fr, IP
Clone
MHR73-11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

RP105/CD180 Antibody (MHR73-11) Summary

Immunogen
This antibody was generated by immunizing host with Human RP105 complexed with mouse MD-1
Localization
B cells, monocytes/macrophages, and dendritic cells
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD180
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
For immunofluorescent staining, the suggested use of this reagent is 2.0 ug per million cells in 100 l volume or 100 l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Cross-Reactivity Baboon, Cynomolgus, Rhesus

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for RP105/CD180 Antibody (MHR73-11)

  • CD180 antigenMGC126233
  • CD180 molecule
  • CD180
  • Ly64
  • LY64MGC126234
  • Ly78
  • Lymphocyte antigen 64
  • lymphocyte antigen-64, radioprotective, 105kDa
  • Radioprotective 105 kDa protein
  • RP105
  • RP105lymphocyte antigen 64 (mouse) homolog, radioprotective, 105kD

Background

CD180, also known as RP105, is a 105 kD member of the toll-like receptor (TLR) family and is expressed on most B cells, monocytes/macrophages and dendritic cells. The TLR family's structural characteristics are critically involved in protein-protein signaling interactions leading to the activation of the innate immune system. Additionally, MD-1, a secretory protein, associates with CD180 (RP105) at the cell surface and this complex, in conjunction with TLR4, recognizes bacterial LPS. The human CD180 (RP105) gene maps to 5q12. The MHR73-11 antibody is able to activate B cells and to protect against irradiation- or dexamethasone-induced apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CD180
Entrez