ROBO1 Antibody

Images

 
Western Blot: Robo1 Antibody [NB100-53804] (0.05ug/ml) staining of fetal Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ROBO1 Antibody [NB100-53804] - Staining of paraffin embedded Mouse Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ROBO1 Antibody Summary

Immunogen
Peptide with sequence C-QKARPAKKQKHQ corresponding to internal region according to Uniprot Mouse NP_062286.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ROBO1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.05 - 0.2 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 200 kDa band observed in fetal mouse brain lysates (calculated MW of 176 kDa band according to NP_062286.2).
Publications
Read Publication using NB100-53804.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for ROBO1 Antibody

  • DUTT1
  • DUTT1Drosophila) homolog 1
  • H-Robo-1
  • MGC131599
  • MGC133277
  • ROBO1
  • roundabout, axon guidance receptor, homolog 1 (Drosophila)

Background

Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. In Drosophila, the roundabout (robo) gene, a member of the immunoglobulin gene superfamily, encodes an integral membrane protein that is both an axon guidance receptor and a cell adhesion receptor for the SLIT1 and SLIT2 proteins. This receptor is involved in the decision by axons to cross the central nervous system midline. Robo1 is structurally similar to the Drosophila roundabout protein. Robo1 is a widely expressed protein, with the exception of the kidney. Robo1 may be required for lung development and defects in this protein may be a cause of breast and lung cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ROBO1 Antibody (NB100-53804)(1)

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Bioinformatics

Gene Symbol ROBO1
Entrez
Uniprot