RNF34 Antibody

Images

 
Western Blot: RNF34 Antibody [NBP1-77904] - HeLa total cell lysate with DR4 antibody at 1ug/mL.
Immunocytochemistry/ Immunofluorescence: RNF34 Antibody [NBP1-77904] - Jurkat cells with DR4 antibody at 10 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

RNF34 Antibody Summary

Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of human DR4 protein.
Specificity
This antibody is directed against human DR4 protein. The antibody is human, mouse and rat reactive. Reactivity against homologues from other sources has not been determined.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RNF34
Purity
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
HeLa whole cell lysate can be used as a positive control for western blotting, and a band at approximately 57 kDa is expected.

Reactivity Notes

Reactive in human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.02% Sodium Azide
Purity
Ion exchange chromatography

Alternate Names for RNF34 Antibody

  • CARP1
  • Caspase regulator CARP1
  • Caspases-8 and -10-associated RING finger protein 1
  • E3 ubiquitin-protein ligase RNF34
  • EC 6.3.2
  • EC 6.3.2.-
  • FLJ21786
  • FYVE-RING finger protein Momo
  • hRFI
  • Human RING finger homologous to inhibitor of apoptosis protein
  • RFI
  • RIF
  • RIFF
  • ring finger protein 34CARP-1
  • RING finger protein RIFF

Background

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines in the TNF family, including TNF and Fas ligand, through their death domain containing receptors, TNFR1 and Fas. A novel death domain containing receptor was recently identified and designated DR4 (for death receptor 4) (1). The ligand for this novel death receptor has been identified and termed TRAIL (2,3), which is a new member in the TNF family. DR4 is also called TRAIL receptor-1 (TRAIL-R1) (4). DR4 is expressed in most human tissues including spleen, peripheral blood leukocytes, small intestine and thymus. Like TNFR1, Fas and DR3, DR4 mediates apoptosis and NF-'B activation in many tissues and cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RNF34
Uniprot