RNF113B Antibody (OTI7A1)

Western Blot: RNF113B Antibody (7A1) [NBP2-01644] Analysis of extracts (35ug) from 9 different cell lines by using anti-RNF113B monoclonal antibody.

Immunocytochemistry/Immunofluorescence: RNF113B Antibody (7A1) [NBP2-01644] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF113B.

Immunohistochemistry-Paraffin: RNF113B Antibody (7A1) [NBP2-01644] - Staining of paraffin-embedded Human breast tissue using anti-RNF113B mouse monoclonal antibody.

Western Blot: RNF113B Antibody (7A1) [NBP2-01644] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF113B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF113B.

Immunohistochemistry-Paraffin: RNF113B Antibody (7A1) [NBP2-01644] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RNF113B mouse monoclonal antibody.

Immunohistochemistry-Paraffin: RNF113B Antibody (7A1) [NBP2-01644] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RNF113B mouse monoclonal antibody.

Immunohistochemistry-Paraffin: RNF113B Antibody (7A1) [NBP2-01644] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RNF113B mouse monoclonal antibody.

Immunohistochemistry-Paraffin: RNF113B Antibody (7A1) [NBP2-01644] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-RNF113B mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm
• Applications: WB, ICC/IF, IHC
• Clone: OTI7A1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.43 mg/ml

RNF113B Antibody (OTI7A1) Summary

• Description: Novus Biologicals Mouse RNF113B Antibody (OTI7A1) (NBP2-01644) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human RNF113B(NP_849192) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RNF113B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 36.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.43 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RNF113B Antibody (OTI7A1)

• bA10G5.1
• MGC26599
• ring finger protein 113B
• Zinc finger protein 183-like 1RNF161ZNF183L1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RNF113B
• Entrez:
  • 140432(Human)