RIC8A Antibody (3G3)

Images

 
Western Blot: RIC8 Antibody (3G3) [NBP2-02433] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIC8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunocytochemistry/ Immunofluorescence: RIC8 Antibody (3G3) [NBP2-02433] Staining of COS7 cells transiently transfected by pCMV6-ENTRY RIC8.
Immunohistochemistry-Paraffin: RIC8 Antibody (3G3) [NBP2-02433] - Staining of paraffin-embedded Kidney tissue using anti-RIC8 mouse monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: RIC8 Antibody (3G3) [NBP2-02433] - Immunofluorescent staining of HepG2 cells using anti-RIC8 mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
3G3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

RIC8A Antibody (3G3) Summary

Immunogen
Full length human recombinant protein of human RIC8A (NP_068751) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RIC8A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RIC8A Antibody (3G3)

  • RIC8
  • RIC8A resistance to inhibitors of cholinesterase 8 homolog A (C. elegans)
  • synembryn
  • synembryn-A

Background

Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Diseases for RIC8A Antibody (NBP2-02433)

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Bioinformatics

Gene Symbol RIC8A
Entrez