Ribosomal Protein S6/RPS6 [p Ser235] Antibody

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Western Blot: Ribosomal Protein S6/RPS6 [p Ser235] Antibody [NBP1-51442] - Analysis of p-RPS6 (S235) pAb at 1:500 dilution Lane1:HEK293T cell lysate treated with colchicine(0.2ng/ml,24h) Lane2:sp2/0 cell lysate treated ...read more
Western Blot: Ribosomal Protein S6/RPS6 [p Ser235] Antibody [NBP1-51442] - Analysis of p-Ribosomal Protein s6 pAb in extracts from K562 TNF cells.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-51442
    • Availability
      Product Discontinued

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Ribosomal Protein S6/RPS6 [p Ser235] Antibody Summary

Immunogen
Synthetic phosphopeptide derived from human Ribosomal Protein S6 around the phosphorylation site of Serine 235.
Modification
p Ser235
Specificity
p-Ribosomal Protein S6 (S235) pAb detects endogenous levels of Ribosomal Protein S6 protein only when phosphorylated at S235.
Clonality
Polyclonal
Host
Rabbit
Gene
RPS6
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
Application Notes
Western blot (WB) analyzes of p-Ribosomal Protein S6 (S235) pAb in extracts from K562 TNF cells.
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ribosomal Protein S6/RPS6 [p Ser235] Antibody

  • Phosphoprotein NP33, 40S ribosomal protein S6
  • pS6
  • Ribosomal Protein S6
  • RPS6
  • S6

Background

The genes encoding for mammalian ribosomal proteins comprise multigene families that consist predominantly of multiple processed pseudogenes and one functional intron-containing gene within their coding regions. The RPS6 gene gives rise to Ribosomal Protein S6 (also designated RPS6). RPS6 is the major substrate of protein kinases in eukaryotic ribosomes. Sequence comparison has identified RPS6 as the equivalent of the Ribosomal Protein S10 from Saccharomyces cerevisiae. The sequence comparison of ribosomal proteins from evolutionarily distant eukaryotes, such as yeast and human, indicates that the structure and probably the function of RPS6 has been highly conserved. RPS6 phosphorylation is stimulated by growth factors, tumor promoting agents and mitogens. It is dephosphorylated at growth arrest.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol RPS6
Entrez