Ribosomal Protein S6/RPS6 Antibody

Images

 
Western Blot: Ribosomal Protein S6/RPS6 Antibody [NB100-92223] - Extracts from raw264.7 cells.
Immunohistochemistry: Ribosomal Protein S6/RPS6 Antibody [NB100-92223] - RS6 (L234) antibody in paraffin-embedded human skeletal muscle tissue.
Western Blot: Ribosomal Protein S6/RPS6 Antibody [NB100-92223] - RS6 (L234) antibody in extracts from HeLa cell treated with TNF-a 20ng/ml 2'.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-92223
    • Availability
      Product Discontinued

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Ribosomal Protein S6/RPS6 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 204-254 of Human Ribosomal Protein S6.
Clonality
Polyclonal
Host
Rabbit
Gene
RPS6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of RS6 (L234) pAb in extracts from raw264.7 cells.
Immunohistochemistry (IHC) analyzes of RS6 (L234) pAb in paraffin-embedded human skeletal muscle tissue.
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross reacts with Human, Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ribosomal Protein S6/RPS6 Antibody

  • Phosphoprotein NP33, 40S ribosomal protein S6
  • pS6
  • Ribosomal Protein S6
  • RPS6
  • S6

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol RPS6
Entrez