Ribophorin I Antibody (3H3)

Images

 
Western Blot: Ribophorin I Antibody (3H3) [NBP2-01294] - Staining of paraffin-embedded Human pancreas tissue using anti-Ribophorin I mouse monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Ribophorin I Antibody (3H3) [NBP2-01294] - expression of RPN1 on pca cell lines vs normal. Image from verified customer review.
Immunohistochemistry-Paraffin: Ribophorin I Antibody (3H3) [NBP2-01294] - Staining of paraffin-embedded Human liver tissue using anti-Ribophorin I mouse monoclonal antibody.
Western Blot: Ribophorin I Antibody (3H3) [NBP2-01294] Analysis of extracts (35ug) from 9 different cell lines by using anti-Ribophorin I monoclonal antibody.
Western Blot: Ribophorin I Antibody (3H3) [NBP2-01294] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Ribophorin I (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of ...read more
Immunohistochemistry-Paraffin: Ribophorin I Antibody (3H3) [NBP2-01294] - Staining of paraffin-embedded Human endometrium tissue using anti-Ribophorin I mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Ribophorin I Antibody (3H3) [NBP2-01294] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Ribophorin I mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Ribophorin I Antibody (3H3) [NBP2-01294] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Ribophorin I mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Ribophorin I Antibody (3H3) [NBP2-01294] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Ribophorin I mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Ribophorin I Antibody (3H3) [NBP2-01294] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Ribophorin I mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
3H3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Ribophorin I Antibody (3H3) Summary

Immunogen
Full length human recombinant protein of human RPN1(NP_002941) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RPN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150
Application Notes
ICC data from customer review.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-01294 in the following applications:

Publications
Read Publication using
NBP2-01294 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human, Dog, Monkey

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Ribophorin I Antibody (3H3)

  • DKFZp686B16177
  • dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit
  • Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit
  • dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
  • EC 2.4.1.119
  • oligosaccharyltransferase 1 homolog
  • OST1
  • ribophorin IRBPH1
  • ribophorin-1
  • RPN-I

Background

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ribophorin I Antibody (NBP2-01294)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
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Review for Ribophorin I Antibody (NBP2-01294) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ICC.

Reviews using NBP2-01294:
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ICC
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Ribophorin I NBP2-01294
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reviewed by:
Anonymous
ICC Human 07/01/2014
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Summary

ApplicationImmunocytochemistry
Sample TestedHuman prostate cells tumor and non tumor
SpeciesHuman

Blocking

Blocking Details5% goat serum

Primary Anitbody

Dilution Ratio1/200 dilution, incubation-Over night at 4 degree

Secondary Antibody

Secondary DescriptionGoat anti mouse alexa flour 488 molecular probes (1/2000)
Secondary Concentration1/2000

Details

Detection Notes172 ms exposure time, Leica DMi3000b
Fixation Detailsfixed using 100% methnol at -20C
Wash Description5 minutes wash with PBS tween

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ribophorin I Antibody (NBP2-01294) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Ribophorin I Antibody (NBP2-01294)

Discover related pathways, diseases and genes to Ribophorin I Antibody (NBP2-01294). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Ribophorin I Antibody (NBP2-01294)

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Pathways for Ribophorin I Antibody (NBP2-01294)

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PTMs for Ribophorin I Antibody (NBP2-01294)

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
07/01/2014
Application: ICC
Species: Human

Bioinformatics

Gene Symbol RPN1
Entrez