RHOXF2B Antibody (RB39659)

Fluorescent confocal image of A549 cell stained with RHOXF2B Antibody (Center).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RHOXF2B primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor� 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37?).Cytoplasmic actin was counterstained with Alexa Fluor� 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37?). Nuclei were counterstained with De) (10 �g/ml, 10 min).RHOXF2B immunoreactivity is localized to Nucleus significantly.

Fluorescent confocal image of A549 cell stained with RHOXF2B Antibody (Center).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RHOXF2B primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor� 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37?).Cytoplasmic actin was counterstained with Alexa Fluor� 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37?). Nuclei were counterstained with De) (10 �g/ml, 10 min).RHOXF2B immunoreactivity is localized to Nucleus significantly.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: RB39659
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

RHOXF2B Antibody (RB39659) Summary

• Immunogen: This RHOXF2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-121 amino acids from the Central region of human RHOXF2B.(Accession#:NP_001093155.1)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RHOXF2B
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Antigen and protein A Affinity-purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RHOXF2B