RhoGDI Antibody (2G3)

Images

 
Western Blot: RhoGDI Antibody (2G3) [NBP2-37472] - Western blot analysis using ARHGDIA mouse mAb against Jurkat (1), HeLa (2), NIH3T3 (3), C6 (4), K562 (5), and COS7 (6) cell lysate.
Immunocytochemistry/ Immunofluorescence: RhoGDI Antibody (2G3) [NBP2-37472] - Immunofluorescence analysis of HepG2 cells using ARHGDIA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry: RhoGDI Antibody (2G3) [NBP2-37472] - Flow cytometric analysis of HeLa cells using ARHGDIA mouse mAb (green) and negative control (red).
Western Blot: RhoGDI Antibody (2G3) [NBP2-37472] - Western blot analysis using ARHGDIA mAb against human ARHGDIA recombinant protein. (Expected MW is 48.7 kDa)
ELISA: RhoGDI Antibody (2G3) [NBP2-37472] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, CyTOF-ready
Clone
2G3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

RhoGDI Antibody (2G3) Summary

Immunogen
Purified recombinant fragment of human ARHGDIA (AA: FULL(1-204)) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ARHGDIA
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • CyTOF-ready
Application Notes
This antibody is Cytof ready.
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for RhoGDI Antibody (2G3)

  • GDIA1
  • MGC117248
  • Rho GDI 1
  • Rho GDP dissociation inhibitor (GDI) alpha
  • rho GDP-dissociation inhibitor 1
  • Rho-GDI alpha
  • RHOGDI
  • RHOGDI-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ARHGDIA