RhoA Antibody

Images

 
Western Blot: RhoA Antibody [NB100-92263] - Western blot (WB) analysis of RhoA (R182) antibody in extracts from HepG2 cells.
Immunohistochemistry: RhoA Antibody [NB100-92263] - Immunohistochemistry (IHC) analyzes of RhoA (R182) antibody in paraffin-embedded human breast carcinoma tissue.

Product Details

Summary
Product Discontinued
View other related RhoA Primary Antibodies

Order Details


    • Catalog Number
      NB100-92263
    • Availability
      Product Discontinued

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RhoA Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 150-200 of Human Rho A.
Clonality
Polyclonal
Host
Rabbit
Gene
RHOA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of RhoA (R182) pAb in extracts from HepG2 cells.
Immunohistochemistry (IHC) analyzes of RhoA (R182) pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RhoA Antibody

  • h12
  • oncogene RHO H12
  • ras homolog gene family, member A
  • Rho cDNA clone 12
  • RHO12
  • RhoA
  • RHOH12ARH12ARHAAplysia ras-related homolog 12
  • small GTP binding protein RhoA
  • transforming protein RhoA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RhoA Antibody (NB100-92263) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for RhoA Antibody (NB100-92263)

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Research Areas for RhoA Antibody (NB100-92263)

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Blogs on RhoA.

The use of actin as a loading control in research on fruiting-body development and vegetative growth in Sordaria macrospora research
Sordaria macrospora is a filamentous fungus that serves as very useful system for scientific research due to a short life cycle and easy manipulation.  Just like any other model organism, it is important to have an effective loading control to va...  Read full blog post.

HIF-1 beta - activating gene transcription in response to hypoxia
Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric transcription factor consisting of alpha and beta subunits. The levels of functional HIF-1 in the cell depends on the level of oxygen allowing cells to respond to hypoxic conditions. HIF-1a is a...  Read full blog post.

A good helper on validating your FLOW and IHC data - Rabbit IgG Isotype Control
Isotype controls are primarily used as negative controls in flow cytometry but they can also be used for immunohistochemistry. They are used to approximate the non-specific target primary antibody binding due to protein-protein interactions, binding t...  Read full blog post.

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Bioinformatics

Gene Symbol RHOA
Entrez