RFX5 Overexpression Lysate (Denatured)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

RFX5 Overexpression Lysate (Denatured) Summary

Description
Pasmid: pCMV-RFX5 full-length
Gene
RFX5

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, and 0.01% Bromophenol blue).

Lysate Details for RFX5

Type
Overexpression
Protein State
Denatured

Notes

Quality control test: Transient overexpression cell lysate was tested with Anti-RFX5 antibody by Western Blots.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RFX5 Overexpression Lysate (Denatured)

  • DNA-binding protein RFX5
  • Regulatory factor X 5
  • regulatory factor X, 5 (influences HLA class II expression)

Background

A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for RFX5 Lysate (H00005993-T01) (0)

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Video Protocols

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Bioinformatics

Gene Symbol RFX5