RFC2 Antibody

Images

 
Western Blot: RFC2 Antibody [NB100-232] - Nuclear extract (50 ug) from HeLa cells. Antibody used at 1 ug/ml.
Western Blot: RFC2 Antibody [NB100-232]
Proximity Ligation Assay: RFC2 Antibody [NB100-232] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC2 (NB100-232) and rabbit anti-human E2F4 (NBP1-21374). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: RFC2 Antibody [NB100-232] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC2 (NB100-232) and rabbit anti-human phospho RAD 17 [S645] (NB100-273). Image merged from DAPI ...read more
Proximity Ligation Assay: RFC2 Antibody [NB100-232] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC2 (NB100-232) and rabbit anti-human RFC1 (NB600-961). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: RFC2 Antibody [NB100-232] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC2 (NB100-232) and rabbit anti-human BRCA1 (NB100-61617). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: RFC2 Antibody [NB100-232] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC2 (NB100-232) and rabbit anti-human DNA-PKcs (NB100-658). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: RFC2 Antibody [NB100-232] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC2 (NB100-232) and rabbit anti-human RFC4 (NB100-403). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: RFC2 Antibody [NB100-232] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC2 (NB100-232) and rabbit anti-human RAD9 (NB110-38891). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: RFC2 Antibody [NB100-232] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC2 (NB100-232) and rabbit anti-human RFC3 (NB100-435). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: RFC2 Antibody [NB100-232] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC2 (NB100-232)and rabbit anti-human PCNA (NB100-456). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: RFC2 Antibody [NB100-232] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC2 (NB100-232) and rabbit anti-human PMS2 (NB100-209). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: RFC2 Antibody [NB100-232] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC2 (NB100-232) and rabbit anti-human CTF18 (NBP1-03346). Image merged from DAPI (2ms) and Texas ...read more

Product Details

Summary
Product Discontinued
View other related RFC2 Primary Antibodies

Order Details


    • Catalog Number
      NB100-232
    • Availability
      Product Discontinued

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RFC2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 175 and 225 of human Replication Factor C subunit 2 using the numbering given in entry NP_852136.1 (GeneID 5982).
Clonality
Polyclonal
Host
Goat
Gene
RFC2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
  • Proximity Ligation Assay 1:1000-1:10000
Application Notes
This antibody can be used for Western Blot. *The investigator should determine the optimal dilution for a specific application.
Positive Control
RFC2 Lysate (NBL1-15292)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RFC2 Antibody

  • A1 40 kDa subunit
  • A1
  • Activator 1 40 kDa subunit
  • Activator 1 subunit 2
  • MGC3665
  • replication factor C (activator 1) 2, 40kDa
  • replication factor C 2
  • Replication factor C 40 kDa subunit
  • replication factor C subunit 2
  • RF-C 40 kDa subunit
  • RFC40replication factor C (activator 1) 2 (40kD)

Background

The Replication factor C (RFC) heteropentamer includes RFC1, RFC2, RFC3, RFC4, and RFC5. The RFC heteropentamer is part of the multiprotein DNA replicase that functions to replicate DNA prior to cell division and repair DNA damage. RFC is the clamp loader ATPase associated with the DNA replicase. As the clamp loader, RFC uses ATP to recruit, open, and close the PCNA ring to link it to DNA for replication and repair. RFC2 is the 40 kDa subunit of RFC that is part of the core complex formed with RFC4 and RFC5. RFC2 is also known as replication factor C subunit 2, replication factor C 40 kDa subunit, RF-C 40 kDa subunit, RFC40, activator 1 40 kDa subunit, A1 40 kDa subunit, A1, and MGC3665.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for RFC2 Antibody (NB100-232). (Showing 1 - 1 of 1 FAQ).

  1. What is the sodium azide percentage for this product?
    • 0.1% sodium azide 

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional RFC2 Products

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Diseases for RFC2 Antibody (NB100-232)

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Bioinformatics

Gene Symbol RFC2
Entrez
Uniprot