Retinoic Acid Receptor gamma 1 Antibody

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Western Blot: Retinoic Acid Receptor gamma 1 Antibody [NB120-5904] - WB detection of RAR gamma 1 from human HEK293 cells. Lane 1 shows NB120-5904 incubated with the neutralizing peptide, lane 2 shows RAR detection by ...read more

Order Details


    • Catalog Number
      NB120-5904
    • Availability
      Product Discontinued

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Retinoic Acid Receptor gamma 1 Antibody Summary

Immunogen
The immunizing peptide corresponds to the N-terminus of RAR gamma-1. This sequence is completely conserved in both mouse and human RAR gamma-1.
Localization
Nuclear
Specificity
This detects RAR gamma-1, but not RAR gamma-2, or either of the alpha or beta forms of RAR.
Clonality
Polyclonal
Host
Rabbit
Gene
RARG
Purity
Unpurified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Gel Super Shift Assays 1 - 2 ul
  • Immunoprecipitation 1:10 - 1:500
Application Notes
GSA: Use 1-2ul. ab5904 has been shown to efficiently super shift the human RAR gamma-1/retinoid X receptor (RXR)/RAR response element complex in gel super shift experiments using a number of different response elements.IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa) representing RAR gamma-1 from transiently transfected 293T cells.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human, Mouse and Rabbit.Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Whole antisera with PBS
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for Retinoic Acid Receptor gamma 1 Antibody

  • NR1B3RARC
  • Nuclear receptor subfamily 1 group B member 3
  • RAR-gamma
  • retinoic acid receptor gamma
  • retinoic acid receptor, gamma

Background

Retinoic acid, a metabolite of vitamin A, is necessary for normal organogenesis but acts as a teratogen at high levels during embryonic and fetal development. Retinoic acid functions through its interaction with the nuclear protein, retinoic acid receptor (RAR). RAR belongs to the steroid and thyroid hormone superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. RAR exists as three major subtypes: alpha, beta and gamma. Two main isoforms of RAR gamma exist: RAR gamma-1 has been found to be predominantly expressed in skin, while RAR gamma-2 has been found early in embryogenesis and in embryonal carcinoma cells. RAR gamma is also known as RARD. The corresponding gene for the retinoic acid receptor gamma 1 is NR1B3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol RARG
Entrez