Reticulum Cells Antibody (ED10)

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications IHC, IHC-Fr
Clone
ED10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Reticulum Cells Antibody (ED10) Summary

Immunogen
Rat spleen cell homogenate
Specificity
NB100-64648 recognizes reticular elements in the T cell areas of spleen, lymph nodes, Peyers patches, bronchus and gut associated lymphoid tissues (BALT and GALT). In the thymus a reticular pattern is observed in capsule and medulla (perivascular). Non-lymphoid organs: in liver, lung and small intestine, some ED10 positive reticular are found. No reaction is observed in skin, kidney, brain, pancreas and heart.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:400-1:1000

Reactivity Notes

Reacts with Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.09% Sodium Azide
Purity
Unpurified

Background

Dendritic cells (DCs) are known as the professional antigen presenting cells. DCs perform the important role of capturing and processing antigen and presenting it to native T-helper cells to initiate the immune response. Distinct subsets of DCs have been described, they include interdigitating reticulum cells. The antigen recognized by NB100-64648 is found on a subpopulation (approximately 5%) of circulating monocytes and peritoneal macrophages, as well as on in vitro LPS and IFN stimulated macrophages and on all macrophages after 3 weeks in culture. It is absent from all other blood cells. Detects a subpopulation of reticulum cells in T-cell areas of lymph nodes and spleen and their in vitro correlates. It is also useful for the detection of a macrophage subpopulation in the thymus. A minor but distinct population of leukocytes in the kidney cortex is also positively stained by ab15683. The antigen is absent in the skin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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