Renal Cell Carcinoma (gp200) Antibody (66.4.C2 (PN-15)) [PerCP]

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    • Catalog Number
      NBP2-33152PCP
    • Availability
      Product Discontinued

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Renal Cell Carcinoma (gp200) Antibody (66.4.C2 (PN-15)) [PerCP] Summary

Immunogen
Fresh, normal human renal cortical tissue homogenate was used as immunogen to generate the RCC antibody.
Localization
Cell surface and cytoplasmic
Specificity
Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
LY75
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Renal Cell Carcinoma (gp200) Antibody (66.4.C2 (PN-15)) [PerCP]

  • CD205LY-75
  • CLEC13BLy-75
  • C-type lectin domain family 13 member B
  • DEC-205CD205 antigen
  • GP200-MR6
  • lymphocyte antigen 75

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol LY75