RelA/NFkB p65 Antibody

Product Discontinued

RelA/NFkB p65 Antibody Summary

• Immunogen: Synthesized non-phosphopeptide derived from human NF-KappaB p65 around the phosphorylation site of serine 276 R-P-S(p) -D-R
• Specificity: Rabbit Anti-NF-KappaB p65 (Ab-276) Polyclonal Antibody detects endogenous levels of total NF-KappaB p65 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RELA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000

Reactivity Notes

Human, mouse, rat

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg 2+ and Ca 2+), pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RelA/NFkB p65 Antibody

• MGC131774
• nf kb p65
• NFkB p65
• NF-kB p65
• NFKB3
• NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65))
• Nuclear factor NF-kappa-B p65 subunit
• Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65
• p65
• p65RelA
• rela p65
• RelA
• v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65

Background

NFkB is formed through the association of multiple subunits, either as a homodimer or heterodimer. Subunits have been identified as p50 (NFkB1), p65 (RelA), c-Rel, RelB and p52 (NFkB2). The classic NFkB form exists as a p50-p65 heterodimer and predominates in many cell types. Many of the possible combinatorial forms of homo- and heterodimers have been identified and growing evidence indicates that different forms of NFkB have different functions in cells. Interestingly, both the p50 and p52 subunits are derived from the precursor proteins p105 and p100 respectively, that each contain multiple copies of the so called ankyrin repeat at their C termini. Nuclear translocation of NFkB is confirmed by the use of electrophorectic mobility shift assays or by immunoblotting with nuclear extracts. The subunit composition of NFkB is confirmed by the use of antibodies that "supershift" the DNA/protein complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RelA/NFkB p65 Antibody (NBP1-48427)(1)

Publication using NBP1-48427

• Chuang SM, Lu JH, Lin KL et al. Epigenetic regulation of COX2 expression by DNA hypomethylation via NF-kappa B activation in ketamine induced ulcerative cystitis Int. J. Mol. Med. 2019-06-21 [PMID: 31257475] (WB, Rat)

Bioinformatics

• Gene Symbol: RELA
• Entrez:
  • 5970(Human)
• Uniprot:
  • Q04206()