RelA/NFkB p65 [p Ser468] Antibody

Images

 
Western Blot: NFkB p65 [p Ser468] Antibody [NB100-92593] - Western blot (WB) analysis ofNFkB p65 [p Ser468] Antibody in extracts from HeLa cells.
Immunohistochemistry: NFkB p65 [p Ser468] Antibody [NB100-92593] - Immunohistochemistry (IHC) analyzes of NFkB p65 [p Ser468] Antibody in paraffin-embedded human breast carcinoma tissue.

Product Details

Summary
Product Discontinued
View other related RelA/NFkB p65 Primary Antibodies

Order Details


    • Catalog Number
      NB100-92593
    • Availability
      Product Discontinued

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RelA/NFkB p65 [p Ser468] Antibody Summary

Immunogen
Synthetic phosphopeptide derived from human NFkB-p65 around the phosphorylation site of Serine 468.
Modification
p Ser468
Clonality
Polyclonal
Host
Rabbit
Gene
RELA
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:500-1:200
Application Notes
Western blot (WB) analysis of NFkB p65 [p Ser468] pAb in extracts from HeLa cells.
Immunohistochemistry (IHC) analyzes of NFkB p65 [p Ser468] pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
RelA/NFkB p65 Lysate (NBL1-15276)

Reactivity Notes

This antibody is reactive against Human, Mouse,.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RelA/NFkB p65 [p Ser468] Antibody

  • MGC131774
  • NF kB p65 phospho
  • nf kb p65
  • NFkB p65 phospho
  • NF-kB p65 phospho
  • NFkB p65
  • NF-kB p65
  • NFKB3
  • NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65))
  • Nuclear factor NF-kappa-B p65 subunit
  • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65
  • p65
  • p65RelA
  • phospho NF kB p65
  • phospho NFkB p65
  • phospho NF-kB p65
  • rela p65
  • RelA
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65

Background

Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NFkappaB (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp kB sequence in the immunoglobulin kappa light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NFkappaB is activated and rapidly transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct 50 kDa proteins have been described, designated p105 and p100, respectively. The p105 precursor contains p50 at its amino terminus and a C-terminal region that when expressed as a separate molecule, designated PDI, binds to p50 and regulates its activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for RelA/NFkB p65 Antibody (NB100-92593)

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Research Areas for RelA/NFkB p65 Antibody (NB100-92593)

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Blogs on RelA/NFkB p65.

The effect of antioxidants and the NFkB p65 pathway in inflammation
NFkB is a transcription factor that plays a role in the expression of genes involved in immune response, inflammation, metastasis, cell survival and more. RelA (p65) is one member of the NFkB mammalian family, alongside other subunits. NFkB subunit...  Read full blog post.

NFkB3-p65: Say that three times fast!
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes such as inflammation, immunity, differentiation, cell growth, tumor genesis and apoptosis. Unlike the majority of transcription factors that reside in the nucleus...  Read full blog post.

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Bioinformatics

Gene Symbol RELA
Entrez