RelA/NFkB p65 [p Ser529] Antibody

Images

 
Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NB100-82079] - Analysis of extracts from 293 cells untreated or treated with TNF - alpha using NFkB p65 Antibody.
Immunocytochemistry/ Immunofluorescence: RelA/NFkB p65 [p Ser529] Antibody [NB100-82079] - Staining of methanol-fixed Hela cells using NFkB-p65 (Phospho-Ser529) Antibody.
Immunohistochemistry-Paraffin: RelA/NFkB p65 [p Ser529] Antibody [NB100-82079] - Analysis of paraffin-embedded human breast carcinoma tissue, using NF-kappB-p65 (Phospho-Ser529) antibody.
Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NB100-82079] - Analysis of extracts from MDA-MB-231 cells, untreated or treated with TNF-a (20ng/ml, 10min) using NF-kB p65 antibody (Lanes 1 and 2) and NF-kB p65 ...read more
Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NB100-82079] - Analysis of extracts from MCF cells, treated with T NFa+Ca, or calf intestinal phospatase (CIP), using NFkB p65 Antibody.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-82079
    • Availability
      Product Discontinued

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RelA/NFkB p65 [p Ser529] Antibody Summary

Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human NFk-B p65 around the phosphorylation site of serine 529 (L-L-SP-G-D).
Modification
p Ser529
Specificity
NFkB-p65 (specific to Phospho-Ser529) detects endogenous levels of NFkB-p65 protein only when phosphorylated at serine 529
Clonality
Polyclonal
Host
Rabbit
Gene
RELA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
RelA/NFkB p65 Lysate (NBL1-15276)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS [pH 7.4] 150mM NaCl and 50% glycerol (without Mg2+ and Ca2+)
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RelA/NFkB p65 [p Ser529] Antibody

  • MGC131774
  • NF kB p65 phospho
  • nf kb p65
  • NFkB p65 phospho
  • NF-kB p65 phospho
  • NFkB p65
  • NF-kB p65
  • NFKB3
  • NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65))
  • Nuclear factor NF-kappa-B p65 subunit
  • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65
  • p65
  • p65RelA
  • phospho NF kB p65
  • phospho NFkB p65
  • phospho NF-kB p65
  • rela p65
  • RelA
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65

Background

p65 is a subunit of the nuclear factor kappa B. The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory protein known as IkB. An impressive variety of stimuli (tumor necrosis factor, interleukin 1, T cell activation signals, bacterial endotoxins, viral transforming proteins, certain growth factors and reactive oxygen intermediates) lead to the rapid nuclear accumulation of NFkB by the induced phosphorylation and subsequent degradation of IkB. In the nucleus, NFkB regulates genes encoding cytokines, cytokine receptors, cell adhesion molecules, proteins involved in coagulation and genes involved in cell growth control. Additionally, NFkB is thought to be an important transcriptional regulator for HIV. Growing evidence indicates that the dysregulation of NFkB may be key to a number of diseases including arthritis and other inflammatory diseases, Alzheimer's disease, atherosclerosis and cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional RelA/NFkB p65 Products

Bioinformatics Tool for RelA/NFkB p65 Antibody (NB100-82079)

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Diseases for RelA/NFkB p65 Antibody (NB100-82079)

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PTMs for RelA/NFkB p65 Antibody (NB100-82079)

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Research Areas for RelA/NFkB p65 Antibody (NB100-82079)

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Blogs on RelA/NFkB p65.

The effect of antioxidants and the NFkB p65 pathway in inflammation
NFkB is a transcription factor that plays a role in the expression of genes involved in immune response, inflammation, metastasis, cell survival and more. RelA (p65) is one member of the NFkB mammalian family, alongside other subunits. NFkB subunit...  Read full blog post.

NFkB3-p65: Say that three times fast!
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes such as inflammation, immunity, differentiation, cell growth, tumor genesis and apoptosis. Unlike the majority of transcription factors that reside in the nucleus...  Read full blog post.

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Bioinformatics

Gene Symbol RELA
Entrez
Uniprot