Reg3D Antibody (518818) [Unconjugated]

Reg3D was detected in immersion fixed paraffin-embedded sections of mouse pancreas using Rat Anti-Mouse Reg3D Monoclonal Antibody (Catalog # MAB5678) at 15 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005) or the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to pancreatic islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Mu
• Applications: IHC
• Clone: 518818
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Reg3D Antibody (518818) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse Reg3D
  Glu27-Gly175
• Specificity: This antibody detects mouse Reg3D in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Reg1A, 1B, recombinant mouse (rm) Reg1, 2, 3A, 3B, 3G, 4, or recombinant rat Reg3 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Reg3d
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Reg3D Antibody (518818) [Unconjugated]

• INGAPrP
• islet neogenesis associated protein-related protein;Reg III delta;regenerating islet-derived 3 delta
• Reg3D

Background

Reg3D (regenerating islet-derived III delta), also called INGAPrP (islet neogenesis-associated protein-related protein) is an ~20 kDa member of the Reg family of secreted calcium-dependent C-type lectins. Reg proteins are involved in various biological functions, including acting as a survival/growth factors. Reg3D mRNA is expressed mainly in pancreatic islets and the digestive system. The mature 149 amino acid Reg3D shares ~50 - 70% amino acid sequence identity with other mammalian Reg3 proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Reg3D Antibody (MAB5678)(1)

Publication using MAB5678

• J Son, W Du, M Esposito, K Shariati, H Ding, Y Kang, D Accili Genetic and pharmacologic inhibition of ALDH1A3 as a treatment of beta-cell failure Nature Communications, 2023-02-02;14(1):558. 2023-02-02 [PMID: 36732513] (IHC, Mouse)

Bioinformatics

• Gene Symbol: Reg3d