Recoverin Antibody (6A55CD6)

Images

 
Western Blot: Recoverin Antibody (6A55CD6) [NB300-280]-Western blot analysis of Recoverin expression in human brain using NB300-280.

Product Details

Summary
Product Discontinued
View other related Recoverin Primary Antibodies

Order Details


    • Catalog Number
      NB300-280
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

Recoverin Antibody (6A55CD6) Summary

Immunogen
Full length recombinant human Recoverin. [UniProt# P35243]
Localization
Cytoplasmic
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RCVRN
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:500
Application Notes
This Recoverin Antibody (6A55CD6) is useful for Immunocytochemistry/Immunofluorescence and Western blot.
Control
Recoverin Overexpression Lysate
Publications
Read Publication using NB300-280.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
4.5 mg/ml
Purity
Protein G purified

Alternate Names for Recoverin Antibody (6A55CD6)

  • cancer associated retinopathy antigen
  • Protein CAR
  • RCV1Cancer-associated retinopathy protein
  • recoverin

Background

Recoverin (RCVRN; also called Protein CAR), is a cytoplasmic calcium-binding protein which is found mainly in the pineal gland and retina, distributed in photoreceptor cells, bipolar cells, as well as in a small population of cells in ganglion cell layer. In photoreceptor cells, this neuronal calcium sensor protein plays a key role in the recovery phase of phototransduction by regulating activity of rhodopsin kinase (RK). Recoverin is consists of two domains each containing a pair of EF-hand-type Ca2+-binding sites that binds two Ca2+ ions: one with high affinity, and the other with low affinity. Upon binding of Ca2+ ions, recoverin undergoes a series of conformational changes allowing the exposure of amino acid residues of its hydrophobic pocket intended for interaction with RK. In parallel, the myristoyl group of recoverin (N-terminus acylated with myristic acid) becomes exposed imparting the interaction with photoreceptor membranes (so-called Ca2+/myristoyl switch mechanism). In photoreceptor cells, recoverin inhibits RK in dark while this inhibition is relieved upon illumination, and the subsequent drop of intracellular Ca2+ levels. Recoverin allows RK to phosphorylate bleached receptor, rhodopsin, which is the first step to its desensitization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00003208-B01P
Species: Hu
Applications: WB, ICC/IF
NBP1-47602
Species: Hu, Mu, Bv, Vb
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NB120-2776
Species: Hu, Bv
Applications: WB, ICC/IF, IHC, IHC-Fr, IP
NBP1-37003
Species: Hu
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA
PP-N4111-00
Species: Hu
Applications: WB, IHC, IP
NB120-19347
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-01860
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC
NBP1-47935
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-88192
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-85016
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-50028
Species: Hu, Mu, Rt, Bv
Applications: WB, ICC/IF, IHC, IHC-P, IHC-FrFl
NBP2-14871
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NB120-2860
Species: Hu, Mu, Rt, Ba, Bv, Ch
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-98474
Species: Hu
Applications: WB
NBP2-55158
Species: Hu
Applications: IHC, IHC-P
NB300-141
Species: Hu, Mu, Rt, Po, Bv, Ch, Eq, Gp, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NB300-280
Species: Hu
Applications: WB, ICC/IF

Publications for Recoverin Antibody (NB300-280)(1)


Showing Publication 1 - 1 of 1.
Publication using NB300-280 Applications Species
Bazhin AV. Recoverin. Calcium Binding Proteins. 2008,3(1):e1-e3

Reviews for Recoverin Antibody (NB300-280) (0)

There are no reviews for Recoverin Antibody (NB300-280). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Recoverin Antibody (NB300-280) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional Recoverin Products

Bioinformatics Tool for Recoverin Antibody (NB300-280)

Discover related pathways, diseases and genes to Recoverin Antibody (NB300-280). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Recoverin Antibody (NB300-280)

Discover more about diseases related to Recoverin Antibody (NB300-280).
 

Pathways for Recoverin Antibody (NB300-280)

View related products by pathway.

PTMs for Recoverin Antibody (NB300-280)

Learn more about PTMs related to Recoverin Antibody (NB300-280).
 

Research Areas for Recoverin Antibody (NB300-280)

Find related products by research area.

Blogs on Recoverin

There are no specific blogs for Recoverin, but you can read our latest blog posts.
Coronavirus Brochure

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recoverin Antibody (6A55CD6) and receive a gift card or discount.

Bioinformatics

Gene Symbol RCVRN