Recombinant Mouse Wnt-2b Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Catalog# & Formulation Size Price

Recombinant Mouse Wnt-2b Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit cell proliferation/survival assay using C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 1-5 µg/ml.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-2b protein
Ser57-Thr389
Accession #
N-terminal Sequence
Ser57 & Asp71
Protein/Peptide Type
Recombinant Proteins
Gene
Wnt2b
Purity
>95%, by SDS-PAGE with silver staining, under reducing conditions
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
41-60 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
Purity
>95%, by SDS-PAGE with silver staining, under reducing conditions
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Wnt-2b Protein, CF

  • Protein Wnt-13
  • protein Wnt-2b
  • wingless-type MMTV integration site family, member 2B
  • Wnt-13
  • WNT13XWNT2, Xenopus, homolog of
  • Wnt2b
  • Wnt-2b
  • XWNT2
  • XWNT2wingless-type MMTV integration site family, member 13

Background

Mouse Wnt-2b (also known as Wnt-13) is a member of the large and highly conserved Wnt family of signaling molecules that have roles in pattern formation, cell fate decision, axon guidance, and tumor formation (1). Mouse Wnt-2b is 389 amino acids (aa) in length and includes a signal peptide (2). Mature mouse Wnt-2b has a predicted molecular mass of 38 kDa and shares 96% aa sequence identity with the human protein. Wnt-2b initiates B-catenin-mediated canonical signaling and is implicated in the development of the lung, kidney, liver, and eye, as well as being present in ovarian surface epithelial cells (3-8). Pathological expression of Wnt-2b in tumors is correlated with increased cell proliferation and decreased survival (9).
  1. Anastas, J.N. and R.T. Moon (2013) Nat. Rev. Cancer 13:11.
  2. Zakin, L.D. et al. (1998) Mech. Dev. 73:107.
  3. Goss, A.M. et al. (2009) Dev. Cell 17:290.
  4. Ricken, A. et al. (2002) Endocrinology 143:2741.
  5. Lin, Y. et al. (2001) Dev. Dyn. 222:26.
  6. Jasoni, C. et al. (1999) Dev. Dyn. 215:215.
  7. Ober, E.A. et al. (2006) Nature 442:688.
  8. Kubo, F. et al. (2005) Development 132:2759.
  9. Kobayashi, M. et al. (2012) Exp. Ther. Med. 3:952.

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Bioinformatics

Gene Symbol Wnt2b
Entrez
Uniprot