Recombinant Mouse SIGNR7/CD209g Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse SIGNR7/CD209g Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of LS180 human colorectal adenocarcinoma cells. When 5 x 10⁴ cells/well are added to rmSIGN-R7 coated plates (5 μg/mL, 100 μL/well), approximately 70%-90% will adhere after 1 hour at 37° C. Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived mouse SIGNR7/CD209g protein
  Arg71-Tyr255 with an N-terminal 6-His tag
• Accession #: CAL15171
• N-terminal Sequence: 6 His-Arg71
• Protein/Peptide Type: Recombinant Proteins
• Gene: Cd209g
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 21.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 29-30 kDa reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse SIGNR7/CD209g Protein, CF

• CD209g
• SIGNR7

Background

SIGNR7 (dendritic cell-specific ICAM-3 grabbing nonintegrin related gene 7), also known as CD209g, belongs to the family of C-type lectins that participate in innate immune responses by binding and clearing pathogens. SIGNR7 is one of several mouse proteins which are encoded by separate genes and exhibit structural and sequence homology to human DC-SIGN and DC-SIGNR (1 - 3). Mouse SIGNR7 is a type 2 membrane protein that consists of a 45 amino acid (aa) cytoplasmic domain, a 22 aa transmembrane segment, and a 188 aa extracellular domain with a juxtamembrane neck region and a single carbohydrate recognition domain (CRD). Within the CRD, mouse SIGNR7 shares 44% - 49% aa sequence identity with mouse SIGNR1, 2, 3, 4, and 5 and 81% with SIGNR8. It shares 44% - 54% aa sequence identity with the CRD of bovine SIGNR7, human DC-SIGN, human DC-SIGNR, and rat DC-SIGN. A SIGNR7 ortholog has been described in cow but not in human (4). SIGNR7 binds both fucose and mannose residues and shows a distinct binding preference for the 6’-sulfo-sialyl Lewis× carbohydrate (5). Its interaction with mannosylated BSA is disrupted at low pH (5).

1. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
2. Cambi, A. and Figdor, C.G. (2005) Curr. Opin. Immunol. 17:345.
3. Park, C.G. et al. (2001) Int. Immunol. 13:1283.
4. Yamakawa, Y. et al. (2008) J. Leukoc. Biol. 83:1396.
5. Powlesland, A.S. et al. (2006) J. Biol. Chem. 281:20440.

Bioinformatics

• Gene Symbol: Cd209g
• Uniprot:
  • CAL15171(Mouse)