Recombinant Mouse Nope Fc Chimera Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Mouse Nope Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized rmNope/Fc Chimera at 10 µg/mL (100 µL/well) can bind rmNetrin-4 with a linear range of 8-250 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Nope protein
Mouse Nope
(Gly22 - His 953)
Accession # Q9EQS9
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Gly22
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Igdcc4
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
128.2 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
160-170 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Nope Fc Chimera Protein, CF

  • DDM36
  • hDDM36
  • IGDCC4
  • immunoglobulin superfamily DCC subclass member 4
  • immunoglobulin superfamily, DCC subclass, member 4
  • KIAA1628
  • likely ortholog of mouse neighbor of Punc E11
  • LOC57722
  • Neighbor of punc e11
  • Nope
  • NOPEFLJ42051
  • Protein DDM36

Background

Mouse Nope (Neighbor Of Punc E11) was discovered as a gene proximal to the Punc gene on chromosome 9 (1). Punc and Nope are distant members of a subgroup of the immunoglobulin (Ig) superfamily, which include DCC (Deleted in Colorectal Cancer) (2), Caenorhabditis elegans UNC5 (UNC = behaviorally uncoordinated) and its mammalian homologues (rat UNC5H1 and H2, mouse UNC5H2 and H3, and human UNC5H3 and H4) (3), Drosophila Frazzled (4), vertebrate Neogenin (5), and mouse Nope and mouse Punc. Members of this subgroup of the Ig superfamily are type I transmembrane proteins with four Ig domains in their extracellular regions.

Mouse Nope consists of a 21 amino acid (aa) signal peptide, a 933 aa extracellular domain (including four Ig domains, five fibronectin-type III (FnIII) repeats), a 24 aa transmembrane segment, and a 274 aa cytoplasmic domain (1). The extracellular domain of mNope shares 45% aa sequence similarity with mouse Punc. However, the cytoplasmic domains of mNope and mouse Punc do not share aa sequence similarity. Compared to other members of the subgroup of the Ig superfamily, mouse Nope extracellular domain shares 25.8% and 25.2% aa similarity with mouse DCC and mouse Neogenin, respectively. Mouse and human Nope share 90.8% aa sequence similarity. Mouse Nope is expressed mostly in embryonic muscle tissues and in developing and adult nervous systems. The structural similarities between Nope and the guidance receptor of the DCC family suggest that Nope may have similar functions as the DCC family (6 - 8).

  1. Salbaum, J.M. and C. Kappen (2000) Genomics 64:15.
  2. Fearon et al. (1990) Science 247:49.
  3. Keino-Masu et al. (1996) Cell 87:175.
  4. Hong, K. et al. (1999) Cell 97:927.
  5. Leonardo, E.D. et al. (1997) Nature 386:833.
  6. Kolodziej et al. (1996) Cell 87:197.
  7. Meyerhardt (1997) Oncogene 14:1129.
  8. Vielmetter et al. (1997) Genomics 41:414.

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Bioinformatics

Gene Symbol Igdcc4
Uniprot