Recombinant Mouse LRP-6 Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Mouse LRP-6 Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. rmLRP-6 binds rhDkk-1, biotin with an apparent KD <15 nM. Biotinylated rmWnt-3a immobilized on a streptavidin-coated plate at 1 µg/mL can bind rmLRP-6 with a linear range of 0.2-10 µg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse LRP-6 protein
Ala20-Pro1368, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Ala20
Protein/Peptide Type
Recombinant Proteins
Gene
Lrp6
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.1 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
152.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
170 kDa, reducing conditions
Publications
Read Publications using
2960-LR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse LRP-6 Protein, CF

  • ADCAD2
  • FLJ90062
  • FLJ90421
  • low density lipoprotein receptor-related protein 6
  • low-density lipoprotein receptor-related protein 6
  • LRP6
  • LRP-6

Background

The low-density lipoprotein (LDL) receptor-related protein 5 (LRP-5) and LRP-6 constitute a distinct subgroup of the LDL receptor family (1). Both LRP-5 and LRP-6 are type I transmembrane proteins that function as Wnt co-receptors with Frizzled proteins (FZD) (2, 3, 4). The mouse LRP-6 cDNA encodes a 1613 amino acid (aa) residue precursor including a 19 aa signal sequence, 1351 aa extracellular domain (ECD), a 23 aa transmembrane (TM) segment, and a 20 aa cytoplasmic domain (5). The ECD contains 20 YWTD motif-containing LDLR-B domains, fourEGF-like repeats, and three cysteine-rich LDLR-A repeats. The ECD of mouse LRP-6 shares 71% aa sequence identity with the ECD of mouse LRP-5 and 98% aa sequence identity with the ECD of human, rat, and canine LRP-6. The intracellular region of LRP-6 contains repeated PPPSP motifs. When the Ser/Thr in these motifs are phosphorylated, LRP-6 can interact with Axin and propagate canonical Wnt signal transduction (6, 7). LRP-6 forms inactive homodimers via its YWTD-EGF domains (8). Wnt binding to FZD and subsequent association with LRP-6 lead to activating conformational changes in LRP-6 cytoplasmic domains (8). LRP-6 can also interact directly with the Dickkopf (Dkk), sclerostin, and Wise proteins which are modulators of Wnt signaling (9 - 14). Formation of a ternary complex of LRP-6, Dkk-1, and Kremen triggers the internalization of the complex and removal LRP-6 from the cell surface. LRP-5 and LRP-6 share overlapping functions in diverse embryonic developmental processes (15).

  1. Schneider, W.J. and J. Nimpf (2003) Cell Mol Life Sci. 60:892.
  2. He, X. et al. (2004) Development 131:1663.
  3. Seto, E.S. and H.J. Bellen (2004) Trends Cell Biol. 14:45. 
  4. Pinson, K.I. et al. (2000) Nature 407:535. 
  5. Brown, S.D. et al. (1998) Biochem. Biophys. Res. Commun. 248:879.
  6. Tamai, K. et al. (2000) Nature 407:530. 
  7. Tamai, K. et al. (2004) Mol. Cell 13:149. 
  8. Liu, G. et al. (2003) Mol. Cell. Biol. 23:5825.
  9. Mao, B. et al. (2001) Nature 411:321.
  10. Semenov, M.V. et al. (2001) Curr. Biol. 11:951.
  11. Bafico, A. et al. (2001) Nat. Cell Biol. 3:683.
  12. Li, X. et al. (2005) J. Biol. Chem. 280:19883.
  13. Semenov, M. et al. (2005) J. Biol. Chem. 280:26770.
  14. Itasaki, N. et al. (2003) Development 130:4295.
  15. Kelly, O. et al. (2004) Development 131:2803.

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Publications for LRP-6 (2960-LR)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: EnzAct, Surface Plasmon Resonance.


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EnzAct
(1)
Surface Plasmon Resonance
(1)
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(2)
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Bioinformatics

Gene Symbol Lrp6
Entrez
Uniprot