Recombinant Mouse IL-21 R Fc Chimera Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse IL-21 R Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit IL-21-dependent enhancement of IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is 75-500 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse IL-21 R protein
Mouse IL-21 R Subunit
(Cys20 - Pro236)
Accession # Q9JHX3
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Cys20
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Il21r
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
51.5 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
70-80 kDa, reducing conditions
Publications
Read Publications using
596-MR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-21 R Fc Chimera Protein, CF

  • CD360 antigen
  • CD360
  • IL-21 R
  • IL-21 receptor
  • IL21R
  • IL-21R
  • interleukin 21 receptor
  • interleukin-21 receptor
  • MGC10967
  • NILR
  • Novel interleukin receptor

Background

IL-21 R (interleukin-21 receptor) is a type I transmembrane glycoprotein within the class I cytokine receptor family, type 4 subfamily (1 ‑ 5). Complex formation between IL-21 R and the common gamma  chain ( gamma c), also used for IL-2, IL-4, IL-7, IL-9, IL-13 and IL-15 receptors, is required for signaling (6, 7). Mouse IL-21 R cDNA encodes 521 amino acid (aa) including a 19 aa signal peptide, a 218 aa extracellular domain (ECD) with 4 conserved cysteine residues, a fibronectin type III domain, and a WSXWS motif, a 21 aa transmembrane domain and a 271 aa cytoplasmic domain with a Box 1 motif, a kinase domain, and several sites for tyrosine phosphorylation (4, 5). One such site, pY510, mediates STAT binding (1, 2). The mouse IL‑21 R ECD shares 69%, 91%, 65%, 63% and 58% aa identity with human, rat, equine, canine and bovine IL-21 R, respectively. One potential 447 aa isoform, with an alternate start site at aa 83, lacks the four conserved ECD cysteines. IL-21 R is expressed mainly on B cells (highest on mature, activated, follicular and germinal center B cells), NK cells, and activated T cells, but is also found on dendritic cells, alternatively activated macrophages, intestinal lamina propria fibroblasts and epithelial cells, and keratinocytes (1, 3 ‑ 5). Both IL‑21 and IL‑4 are necessary for efficient B cell IgG1 production and normal germinal center architecture (8). B cell IL‑21 R engagement induces Blimp-1 (which mediates plasma cell differentiation), and is important for memory responses (1, 9, 10).  IL-21 R engagement on mouse NK cells enhances their cytotoxic activity and IFN-gamma production (4, 11). IL‑21 R engagement on CD8+ T cells aids control of viral infection and tumor growth; IL-21 R is also necessary for sufficient numbers of regulatory T cells to combat chronic inflammation (1, 12, 13). IL‑21 R expression is often up‑regulated in allergic skin inflammation, systemic lupus erythematosus and diffuse large B cell lymphoma (DLBCL) (1, 2, 14, 15).

  1. Leonard, W.J. et al. (2008) J. Leukoc. Biol. 84:348.
  2. Konforte, D. et al. (2009) J. Immunol. 182:1791.
  3. Monteleone, G. et al., 2009, Cytokine Growth Factor Rev. 20:185.
  4. Parrish-Novak, et al. (2000) Nature 408:57.
  5. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
  6. Asao, H. et al. (2001) J. Immunol. 167:1.
  7. Habib, T. et al. (2002) Biochemistry 41:8725.
  8. Ozaki, K. et al. (2002) Science 298:1630.
  9. Rankin, A.L. et al. (2011) J. Immunol. 186:667.
  10. King, I.L. et al. (2010) J. Immunol. 185:6138.
  11. Kasaian, M.T. et al. (2002) Immunity 16:559.
  12. Frohlich, A. et al. (2009 Science 324:1576.
  13. Tortola, L. et al. (2010) Blood 116:5200.
  14. Jin, H. et al. (2009) J. Clin. Invest. 119:47.
  15. Sarosiek, K.A. et al. (2010) Blood 115:570.

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Publications for IL-21 R (596-MR)(22)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: Binding Assay, Bioassay, Flow, In Vivo.


Filter By Application
Binding Assay
(1)
Bioassay
(6)
Flow
(13)
In Vivo
(2)
All Applications
Filter By Species
Mouse
(22)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using 596-MR Applications Species
KM Valentine, D Davini, TJ Lawrence, GN Mullins, M Manansala, M Al-Kuhlani, JM Pinney, JK Davis, AE Beaudin, SS Sindi, DM Gravano, KK Hoyer CD8 Follicular T Cells Promote B Cell Antibody Class Switch in Autoimmune Disease J. Immunol., 2018;0(0):. 2018 [PMID: 29743314] (Bioassay, Mouse) Bioassay Mouse
JY Choi, A Seth, M Kashgarian, S Terrillon, E Fung, L Huang, LC Wang, J Craft Disruption of Pathogenic Cellular Networks by IL-21 Blockade Leads to Disease Amelioration in Murine Lupus J. Immunol, 2017;0(0):. 2017 [PMID: 28219887] (Flow, Mouse) Flow Mouse
Antigen-presenting cell-derived IL-6 restricts the expression of GATA3 and IL-4 by follicular helper T cells J Leukoc Biol, 2016;0(0):. 2016 [PMID: 27474166] (Flow, Mouse) Flow Mouse
Jang E, Cho W, Oh Y, Cho M, Kim J, Paik D, Youn J Splenic Long-Lived Plasma Cells Promote the Development of Follicular Helper T Cells during Autoimmune Responses. J Immunol, 2016;196(3):1026-35. 2016 [PMID: 26729802] (Bioassay, Mouse) Bioassay Mouse
Wikenheiser D, Ghosh D, Kennedy B, Stumhofer J The Costimulatory Molecule ICOS Regulates Host Th1 and Follicular Th Cell Differentiation in Response to Plasmodium chabaudi chabaudi AS Infection. J Immunol, 2016;196(2):778-91. 2016 [PMID: 26667167] (Flow, Mouse) Flow Mouse
Preite S, Baumjohann D, Foglierini M, Basso C, Ronchi F, Rodriguez B, Corti D, Lanzavecchia A, Sallusto F Somatic mutations and affinity maturation are impaired by excessive numbers of T follicular helper cells and restored by Treg cells or memory T cells. Eur J Immunol, 2015;45(11):3010-21. 2015 [PMID: 26332258] (Flow, Mouse) Flow Mouse
Giles J, Kashgarian M, Koni P, Shlomchik M B Cell-Specific MHC Class II Deletion Reveals Multiple Nonredundant Roles for B Cell Antigen Presentation in Murine Lupus. J Immunol, 2015;195(6):2571-9. 2015 [PMID: 26268653] (Flow,, Mouse) Flow Mouse
Teichmann L, Cullen J, Kashgarian M, Dong C, Craft J, Shlomchik M Local triggering of the ICOS coreceptor by CD11c(+) myeloid cells drives organ inflammation in lupus. Immunity, 2015;42(3):552-65. 2015 [PMID: 25786178] (Flow, Mouse) Flow Mouse
Kim Y, Lim H, Jung H, Wetsel R, Chung Y Regulation of autoimmune germinal center reactions in lupus-prone BXD2 mice by follicular helper T cells. PLoS ONE, 2015;10(3):e0120294. 2015 [PMID: 25768299] (Bioassay, Mouse) Bioassay Mouse
Perez-Mazliah D, Ng D, Freitas do Rosario A, McLaughlin S, Mastelic-Gavillet B, Sodenkamp J, Kushinga G, Langhorne J Disruption of IL-21 signaling affects T cell-B cell interactions and abrogates protective humoral immunity to malaria. PLoS Pathog, 2015;11(3):e1004715. 2015 [PMID: 25763578] (Binding Assay, Mouse) Binding Assay Mouse
Show All 22 Publications.

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Bioinformatics

Gene Symbol Il21r
Entrez
Uniprot