Recombinant Mouse Flt-3 Ligand Protein

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1 μg/lane of Recombinant Mouse Flt-3 Ligand was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing multiple bands between 18-32 kDa. Multiple bands are due to variable ...read more
Recombinant Mouse Flt-3 Ligand (Catalog # 427-FL) stimulates cell proliferation in the BaF3 mouse pro-B cell line transfected with mouse Flt-3. The ED50 for this effect is 0.4-2.4 ng/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse Flt-3 Ligand Protein Summary

Details of Functionality
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with mouse Flt-3. The ED50 for this effect is typically 0.4-2.4 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Flt-3 Ligand protein
Gly27-Arg188
Accession #
N-terminal Sequence
Gly27
Protein/Peptide Type
Recombinant Proteins
Gene
Flt3l
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
Multiple bands between 18-32 kDa, reducing conditions
Publications
Read Publications using
427-FL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Flt-3 Ligand Protein

  • FL
  • Flt3 ligand
  • Flt-3 Ligand
  • Flt3L
  • FLT3LG
  • fms-related tyrosine kinase 3 ligand
  • SL cytokine

Background

Flt-3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages (1-3). Mature mouse Flt‑3 Ligand consists of a 161 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 22 aa cytoplasmic tail (4-6). Within the ECD, mouse Flt-3 Ligand shares 71% and 81% aa sequence identity with human and rat Flt-3 Ligand, respectively. Mouse and human Flt-3 Ligand show cross-species activity (4, 5, 7). Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts (1, 8). Each 36 kDa chain carries approximately 12 kDa of N- and O‑linked carbohydrates (8). Alternate splicing and proteolytic cleavage of the transmembrane form can generate a soluble 30 kDa fragment that includes the cytokine domain (4, 8). Alternate splicing of mouse Flt-3 Ligand also generates a membrane-associated isoform with a 57 aa substitution following the cytokine domain (4, 5, 8, 9). Both transmembrane and soluble Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2 (3 - 6). Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation (2, 10). It synergizes with IL-3, GM‑CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells (4, 5, 7). It cooperates with IL‑2, -6, -7, and -15 to induce NK cell development and with IL-3, -7, and ‑11 to induce terminal B cell maturation (1, 11). Animal studies also show Flt-3 Ligand to reduce the severity of experimentally induced allergic inflammation (12).

  1. Wodnar-Filipowicz, A. (2003) News Physiol. Sci. 18:247.
  2. Dong, J. et al. (2002) Cancer Biol. Ther. 1:486.
  3. Gilliland, D.G. and J.D. Griffin (2002) Blood 100:1532.
  4. Hannum, C. et al. (1994) Nature 368:643.
  5. Lyman, S.D. et al. (1993) Cell 75:1157.
  6. Savvides, S.N. et al. (2000) Nat. Struct. Biol. 7:486.
  7. Lyman, S.D. et al. (1994) Blood 83:2795.
  8. McClanahan, T. et al. (1996) Blood 88:3371.
  9. Lyman, S.D. et al. (1995) Oncogene 10:149.
  10. Diener, K.R. et al. (2008) Exp. Hematol. 36:51.
  11. Farag, S.S. and M.A. Caligiuri (2006) Blood Rev. 20:123.
  12. Edwan, J.H. et al. (2004) J. Immunol. 172:5016.

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427-FL
Species: Mu
Applications: Bioactivity

Publications for Flt-3 Ligand (427-FL)(65)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Cotton Rat.

We have publications tested in 5 applications: Bioassay, Differentiation, ELISA (Standard), ELISA Developmet, In Vivo.


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Bioassay
(58)
Differentiation
(1)
ELISA (Standard)
(1)
ELISA Developmet
(1)
In Vivo
(4)
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Human
(2)
Mouse
(60)
Rat
(2)
Cotton Rat
(1)
All Species
Showing Publications 1 - 10 of 65. Show All 65 Publications.
Publications using 427-FL Applications Species
P Huang, X Li, Y Meng, B Yuan, T Liu, M Jiao, X Wang, Y Liu, H Yin Interleukin-33 regulates hematopoietic stem cell regeneration after radiation injury Stem Cell Res Ther, 2019;10(1):123. 2019 [PMID: 30999922] (Bioassay, Mouse) Bioassay Mouse
N Vannini, V Campos, M Girotra, V Trachsel, S Rojas-Sutt, J Tratwal, S Ragusa, E Stefanidis, D Ryu, PY Rainer, G Nikitin, S Giger, TY Li, A Semilietof, A Oggier, Y Yersin, L Tauzin, E Pirinen, WC Cheng, J Ratajczak, C Canto, M Ehrbar, F Sizzano, TV Petrova, D Vanhecke, L Zhang, P Romero, A Nahimana, S Cherix, MA Duchosal, PC Ho, B Deplancke, G Coukos, J Auwerx, MP Lutolf, O Naveiras The NAD-Booster Nicotinamide Riboside Potently Stimulates Hematopoiesis through Increased Mitochondrial Clearance Cell Stem Cell, 2019;24(3):405-418.e7. 2019 [PMID: 30849366] (Bioassay, Mouse) Bioassay Mouse
S Goossens, J Wang, C Tremblay, J De Medts, S T'Sas, T Nguyen, J Saw, K Haigh, DJ Curtis, P Van Vlierb, G Berx, T Taghon, JJ Haigh ZEB2 and LMO2 drive immature T-cell lymphoblastic leukemia via distinct oncogenic mechanisms Haematologica, 2019;0(0):. 2019 [PMID: 30679322] (Bioassay, Mouse) Bioassay Mouse
F Ribezzo, IAM Snoeren, S Ziegler, J Stoelben, PA Olofsen, A Henic, MV Ferreira, S Chen, USA Stalmann, G Buesche, RM Hoogenboez, R Kramann, U Platzbecke, MHGP Raaijmaker, BL Ebert, RK Schneider Rps14, Csnk1a1 and miRNA145/miRNA146a deficiency cooperate in the clinical phenotype and activation of the innate immune system in the 5q- syndrome Leukemia, 2019;0(0):. 2019 [PMID: 30651631] (Bioassay, Mouse) Bioassay Mouse
F Boscolo Se, D Fox, A Sacco Muscle Stem Cells Give Rise to Rhabdomyosarcomas in a Severe Mouse Model of Duchenne Muscular Dystrophy Cell Rep, 2019;26(3):689-701.e6. 2019 [PMID: 30650360] (Bioassay, Mouse) Bioassay Mouse
LM Hillyer, B Woodward Acutely malnourished weanling mice administered Flt3 ligand can support a cell-mediated inflammatory response Cytokine, 2019;113(0):39-49. 2019 [PMID: 30539781] (ELISA (Standard), Human) ELISA (Standard) Human
M Inoue, K Okamoto, A Terashima, T Nitta, R Muro, T Negishi-Ko, T Kitamura, T Nakashima, H Takayanagi Arginine methylation controls the strength of ?c-family cytokine signaling in T cell maintenance Nat. Immunol., 2018;19(11):1265-1276. 2018 [PMID: 30323341] (Bioassay, Mouse) Bioassay Mouse
SK Martin, S Fitter, N El Khawank, RH Grose, CR Walkley, LE Purton, MA Ruegg, MN Hall, S Gronthos, ACW Zannettino mTORC1 plays an important role in osteoblastic regulation of B-lymphopoiesis Sci Rep, 2018;8(1):14501. 2018 [PMID: 30266921] (Bioassay, Mouse) Bioassay Mouse
R Gonzalez-Q, A Nguyen, DH Kono, MBA Oldstone, AN Theofilopo, R Baccala Lupus acceleration by a MAVS-activating RNA virus requires endosomal TLR signaling and host genetic predisposition PLoS ONE, 2018;13(9):e0203118. 2018 [PMID: 30199535] (Bioassay, Mouse) Bioassay Mouse
YW Lin, LC Wang, CK Lee, SH Chen Flt3 ligand treatment reduces enterovirus A71 lethality in mice with enhanced B cell responses Sci Rep, 2018;8(1):12184. 2018 [PMID: 30111869] (In Vivo, Mouse) In Vivo Mouse
Show All 65 Publications.

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Bioinformatics

Gene Symbol Flt3l
Uniprot