Recombinant Mouse Flt-3 Ligand Protein


1 μg/lane of Recombinant Mouse Flt-3 Ligand was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing multiple bands between 18-32 kDa. Multiple bands are due to variable more
Recombinant Mouse Flt-3 Ligand (Catalog # 427-FL) stimulates cell proliferation in the BaF3 mouse pro-B cell line transfected with mouse Flt-3. The ED50 for this effect is 0.4-2.4 ng/mL.

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Applications Bioactivity

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Recombinant Mouse Flt-3 Ligand Protein Summary

Details of Functionality
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with mouse Flt-3. The ED50 for this effect is typically 0.4-2.4 ng/mL.
Mouse myeloma cell line, NS0-derived mouse Flt-3 Ligand protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Multiple bands between 18-32 kDa, reducing conditions
Read Publications using
427-FL in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Flt-3 Ligand Protein

  • FL
  • Flt3 ligand
  • Flt-3 Ligand
  • Flt3L
  • FLT3LG
  • fms-related tyrosine kinase 3 ligand
  • SL cytokine


Flt-3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages (1-3). Mature mouse Flt‑3 Ligand consists of a 161 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 22 aa cytoplasmic tail (4-6). Within the ECD, mouse Flt-3 Ligand shares 71% and 81% aa sequence identity with human and rat Flt-3 Ligand, respectively. Mouse and human Flt-3 Ligand show cross-species activity (4, 5, 7). Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts (1, 8). Each 36 kDa chain carries approximately 12 kDa of N- and O‑linked carbohydrates (8). Alternate splicing and proteolytic cleavage of the transmembrane form can generate a soluble 30 kDa fragment that includes the cytokine domain (4, 8). Alternate splicing of mouse Flt-3 Ligand also generates a membrane-associated isoform with a 57 aa substitution following the cytokine domain (4, 5, 8, 9). Both transmembrane and soluble Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2 (3 - 6). Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation (2, 10). It synergizes with IL-3, GM‑CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells (4, 5, 7). It cooperates with IL‑2, -6, -7, and -15 to induce NK cell development and with IL-3, -7, and ‑11 to induce terminal B cell maturation (1, 11). Animal studies also show Flt-3 Ligand to reduce the severity of experimentally induced allergic inflammation (12).

  1. Wodnar-Filipowicz, A. (2003) News Physiol. Sci. 18:247.
  2. Dong, J. et al. (2002) Cancer Biol. Ther. 1:486.
  3. Gilliland, D.G. and J.D. Griffin (2002) Blood 100:1532.
  4. Hannum, C. et al. (1994) Nature 368:643.
  5. Lyman, S.D. et al. (1993) Cell 75:1157.
  6. Savvides, S.N. et al. (2000) Nat. Struct. Biol. 7:486.
  7. Lyman, S.D. et al. (1994) Blood 83:2795.
  8. McClanahan, T. et al. (1996) Blood 88:3371.
  9. Lyman, S.D. et al. (1995) Oncogene 10:149.
  10. Diener, K.R. et al. (2008) Exp. Hematol. 36:51.
  11. Farag, S.S. and M.A. Caligiuri (2006) Blood Rev. 20:123.
  12. Edwan, J.H. et al. (2004) J. Immunol. 172:5016.

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Publications for Flt-3 Ligand (427-FL)(65)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Cotton Rat.

We have publications tested in 5 applications: Bioassay, Differentiation, ELISA (Standard), ELISA Developmet, In Vivo.

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Showing Publications 1 - 10 of 65. Show All 65 Publications.
Publications using 427-FL Applications Species
P Huang, X Li, Y Meng, B Yuan, T Liu, M Jiao, X Wang, Y Liu, H Yin Interleukin-33 regulates hematopoietic stem cell regeneration after radiation injury Stem Cell Res Ther, 2019;10(1):123. 2019 [PMID: 30999922] (Bioassay, Mouse) Bioassay Mouse
N Vannini, V Campos, M Girotra, V Trachsel, S Rojas-Sutt, J Tratwal, S Ragusa, E Stefanidis, D Ryu, PY Rainer, G Nikitin, S Giger, TY Li, A Semilietof, A Oggier, Y Yersin, L Tauzin, E Pirinen, WC Cheng, J Ratajczak, C Canto, M Ehrbar, F Sizzano, TV Petrova, D Vanhecke, L Zhang, P Romero, A Nahimana, S Cherix, MA Duchosal, PC Ho, B Deplancke, G Coukos, J Auwerx, MP Lutolf, O Naveiras The NAD-Booster Nicotinamide Riboside Potently Stimulates Hematopoiesis through Increased Mitochondrial Clearance Cell Stem Cell, 2019;24(3):405-418.e7. 2019 [PMID: 30849366] (Bioassay, Mouse) Bioassay Mouse
S Goossens, J Wang, C Tremblay, J De Medts, S T'Sas, T Nguyen, J Saw, K Haigh, DJ Curtis, P Van Vlierb, G Berx, T Taghon, JJ Haigh ZEB2 and LMO2 drive immature T-cell lymphoblastic leukemia via distinct oncogenic mechanisms Haematologica, 2019;0(0):. 2019 [PMID: 30679322] (Bioassay, Mouse) Bioassay Mouse
F Ribezzo, IAM Snoeren, S Ziegler, J Stoelben, PA Olofsen, A Henic, MV Ferreira, S Chen, USA Stalmann, G Buesche, RM Hoogenboez, R Kramann, U Platzbecke, MHGP Raaijmaker, BL Ebert, RK Schneider Rps14, Csnk1a1 and miRNA145/miRNA146a deficiency cooperate in the clinical phenotype and activation of the innate immune system in the 5q- syndrome Leukemia, 2019;0(0):. 2019 [PMID: 30651631] (Bioassay, Mouse) Bioassay Mouse
F Boscolo Se, D Fox, A Sacco Muscle Stem Cells Give Rise to Rhabdomyosarcomas in a Severe Mouse Model of Duchenne Muscular Dystrophy Cell Rep, 2019;26(3):689-701.e6. 2019 [PMID: 30650360] (Bioassay, Mouse) Bioassay Mouse
LM Hillyer, B Woodward Acutely malnourished weanling mice administered Flt3 ligand can support a cell-mediated inflammatory response Cytokine, 2019;113(0):39-49. 2019 [PMID: 30539781] (ELISA (Standard), Human) ELISA (Standard) Human
M Inoue, K Okamoto, A Terashima, T Nitta, R Muro, T Negishi-Ko, T Kitamura, T Nakashima, H Takayanagi Arginine methylation controls the strength of ?c-family cytokine signaling in T cell maintenance Nat. Immunol., 2018;19(11):1265-1276. 2018 [PMID: 30323341] (Bioassay, Mouse) Bioassay Mouse
SK Martin, S Fitter, N El Khawank, RH Grose, CR Walkley, LE Purton, MA Ruegg, MN Hall, S Gronthos, ACW Zannettino mTORC1 plays an important role in osteoblastic regulation of B-lymphopoiesis Sci Rep, 2018;8(1):14501. 2018 [PMID: 30266921] (Bioassay, Mouse) Bioassay Mouse
R Gonzalez-Q, A Nguyen, DH Kono, MBA Oldstone, AN Theofilopo, R Baccala Lupus acceleration by a MAVS-activating RNA virus requires endosomal TLR signaling and host genetic predisposition PLoS ONE, 2018;13(9):e0203118. 2018 [PMID: 30199535] (Bioassay, Mouse) Bioassay Mouse
YW Lin, LC Wang, CK Lee, SH Chen Flt3 ligand treatment reduces enterovirus A71 lethality in mice with enhanced B cell responses Sci Rep, 2018;8(1):12184. 2018 [PMID: 30111869] (In Vivo, Mouse) In Vivo Mouse
Show All 65 Publications.

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Gene Symbol Flt3l