Recombinant Mouse FGL2 Protein, CF

Images

 
There are currently no images for FGL2/Fibroleukin (5257-FL).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse FGL2 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit anti-CD3-induced proliferation of mouse splenic T lymphocytes. Chan, C.W. et al. (2003) J. Immunol. 170:4036. The soluble Recombinant Mouse FGL2 inhibited the proliferation with a typical ED50 value of 1-4 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse FGL2 protein
Val20-Pro432 & Asp31-Pro432 both with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Val20 & Asp31
Structure / Form
Disulfide-linked homotetramer
Protein/Peptide Type
Recombinant Proteins
Gene
Fgl2
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
47.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
60-80 kDa, reducing conditions
Publications
Read Publication using
5257-FL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse FGL2 Protein, CF

  • FGL2
  • fibrinogen-like 2
  • Fibrinogen-like protein 2
  • Fibroleukin
  • pT49T49
  • T49

Background

FGL2 (fibrinogen-like protein 2), also called fibroleukin, is a 64-70 kDa secreted glycoprotein of the Fibrinogen-like superfamily. It has prothrombinase activity and also promotes T regulatory (Treg) activity (1-6). The mouse FGL2 gene encodes a 432 amino acid (aa) protein that contains a 19 aa signal sequence and a 413 aa mature sequence with a coiled-coil region and a fibronectin C-terminal homology domain or FRED (1, 2). A 260-280 aa FGL2 complex is thought to be a tetramer formed by covalent disulfide linkage of dimers that are asoociated via coiled-coil interactions (2, 3). Mature mouse FGL2 shares 91% aa identity with rat FGL2, and 77-80% aa identity with human, equine, porcine, bovine and canine FGL2. FGL2 appears to have two modes of action. One mode involves its prothrombinase activity, which requires calcium and acidic phospholipids (4). This mode is thought to be active during hepatitis viral infections when FGL2, produced by macrophages in response to IFN-gamma, induces hepatic apoptosis and fibrin deposition (7). In addition, FGL2 produced by endothelial cells in response to TNF-alpha within cardiac xenografts or allografts promotes coagulation during acute vascular rejection (7-9). A second mode of action involves soluble (not phospholipid-associated) FGL2 and is independent of prothrombinase activity (2). Soluble FGL2 is required for Treg function, and directly suppresses DC, T, and B cell immune reactivity; consequently, some FGL2-deficent mice develop autoimmune glomerulonephritis (5, 6). In vitro, soluble FGL2 can skew T cell polarization toward Th2 and inhibit proliferation of stimulated T cells and maturation of DC (6). In pregnancy, fetal trophoblast cells secrete FGL2. The immune suppressive mode of FGL2 may prevent early fetal loss; however, the procoagulant mode is thought to mediate infection-triggered abortion (10).

  1. Koyama, T. et al. (1987) Proc. Natl. Acad. Sci. USA 84:1609.
  2. Marazzi, S. et al. (1998) J. Immunol.161:138.
  3. Olson, G. E. et al. (2004) J. Biol. Chem. 279:51266.
  4. Chan, C. W. Y. et al. (2002) J. Immunol. 168:5170.
  5. Shalev, I. et al. (2008) J. Immunol.180:249.
  6. Chan, C. W. Y. et al. (2003) J. Immunol. 170:4036.
  7. Liu, M. et al. (2006) J. Immunol. 176:7028.
  8. Mendicino, M. et al. (2005) Circulation 112:248.
  9. Ning, Q. et al. (2005) J. Immunol. 174:7403.
  10. Clark, D. A. et al. (2004) Mol. Hum. Reprod. 10:99.

Customers Who Viewed This Item Also Viewed...

NBP1-33320
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
AF2724
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready
6507-IL/CF
Species: Hu
NBP1-89679
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP1-84550
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-75649
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
NBP2-01223
Species: Hu
Applications: WB, Flow, IHC, IHC-P
MAB6799
Species: Hu
Applications: WB, IHC
AF1180
Species: Hu
Applications: WB, Simple Western, IHC, ICC
NBP2-20460
Species: Hu, Mu, Rt
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP, ChIP
5257-FL
Species: Mu
Applications: Bioactivity

Publications for FGL2/Fibroleukin (5257-FL)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

Reviews for FGL2/Fibroleukin (5257-FL) (0)

There are no reviews for FGL2/Fibroleukin (5257-FL). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for FGL2/Fibroleukin (5257-FL) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for FGL2/Fibroleukin (5257-FL)

Discover related pathways, diseases and genes to FGL2/Fibroleukin (5257-FL). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for FGL2/Fibroleukin (5257-FL)

Discover more about diseases related to FGL2/Fibroleukin (5257-FL).
 

Pathways for FGL2/Fibroleukin (5257-FL)

View related products by pathway.

PTMs for FGL2/Fibroleukin (5257-FL)

Learn more about PTMs related to FGL2/Fibroleukin (5257-FL).

Blogs on FGL2/Fibroleukin

There are no specific blogs for FGL2/Fibroleukin, but you can read our latest blog posts.

Customers Who Bought This Also Bought

CD4 Antibody
NBP1-19371
IL-4
6507-IL-010/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse FGL2 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol Fgl2
Uniprot