Recombinant Mouse FGF-23 Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse FGF-23 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human FGF RIIIc. The ED50 for this effect is 0.2‑1.2 µg/mL in the presence of Recombinant Mouse Klotho (Catalog # 1819-KL) and heparin.
Source
Mouse myeloma cell line, NS0-derived mouse FGF-23 protein
Tyr25-Val251 (Arg179Gln), with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Tyr25
Protein/Peptide Type
Recombinant Proteins
Gene
Fgf23
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
26.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
30-32 kDa, reducing conditions
Publications
Read Publications using
2629-FG/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse FGF-23 Protein, CF

  • ADHR
  • FGF23
  • FGF-23
  • fibroblast growth factor 23
  • HPDR2
  • HYPF
  • phosphatonin
  • PHPTC
  • tumor-derived hypophosphatemia inducing factor
  • Tumor-derived hypophosphatemia-inducing factor

Background

Fibroblast growth factor 23 (FGF-23) is a 30 - 32 kDa member of the FGF gene family. Based on its structure, it is further classified as an FGF19 subfamily member. This subfamily includes FGF-19, -21, and -23. Like all other FGF subfamilies, FGF-19 subfamily members contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Unlike other FGF subfamilies, FGF-19 subfamily members exist as highly diffusible molecules that is attributed to poor ECM/heparin sulfate binding (3, 4, 5, 6). The cDNA for mouse FGF-23 predicts a 251 aa polypeptide that contains a 24 aa signal sequence and a 227 aa mature region (7). Mature mouse FGF-23 shows 72% aa identity to human FGF-23 (8). The FGF-19 subfamily shares an unusual receptor configuration. The standard model for FGF signaling requires an FGF:FGFR:heparin sulfate complex. Given FGF-23’s minimal association with heparin, a substitute termed ( alpha -) Klotho has evolved that serves the same function. Although FGF-23 binds to the widely expressed “c” isoforms of FGFR1 and 3 plus FGFR4, Klotho has a restricted distribution that limits FGF-23 activity (10, 11, 12). It should be noted that heparin-dependency has been reported for FGF-19 signaling, and this observation may extend to FGF-23 (13). The FGF-19 subfamily is considered endocrine in nature. All three subfamily members impact some aspect of metabolism and all three are induced by a nuclear receptor heterodimer that includes the retinoid X receptor (14, 15, 16). FGF-23 is considered a phosphatonin; that is, a molecule that reduces circulating plasma phosphate. It is produced by osteocytes and osteoblasts in response to high circulating phosphate levels, elevated parathyroid hormone that induces hypercalcemia, and circulatory volume loading. Upon binding to FGF-23 receptors on renal proximal tubular epithelium, two basic changes are seen. First, the enzyme responsible for generating the active form of vitamin D is suppressed, resulting in decreased levels of bioactive vitamin D. Since vitamin D promotes intestinal phosphate absorption, plasma phosphate declines. Second, the transporters responsible for phosphate resorption on renal epithelium are down regulated, resulting in decreased uptake from urine and again a decline in blood phosphorus (17, 18).

  1. Itoh, N. and D.M. Ornitz (2004) Trends Genet. 20:563. 
  2. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  3. Fukumoto, S. (2007) Endocr. J. Sep 14; [Epub ahead of print].
  4. Huang, X. et al. (2006) Mol. Carcinog. 45:934. 
  5. Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417.
  6. Harmer, N.J. et al. (2004) Biochemistry 43:629.
  7. Yamashita, T. et al. (2000) Biochem. Biophys. Res. Commun. 277:494.
  8. Shimada, T. et al. (2001) Proc. Natl. Acad. Sci. USA 98:6500.
  9. Kato, K. et al. (2006) J. Biol. Chem. 281:18370.
  10. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
  11. Urakawa, I. et al. (2006) Nature 444:770.
  12. Hurosu, H. et al. (2006) J. Biol. Chem. 281:6120.
  13. Wu, X. et al. (2007) J. Biol. Chem. 282:29069.
  14. Moore, D.D. (2007) Science 316:1436.
  15. Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci. USA 104:7432.
  16. Kurosu, H. et al. (2007) J. Biol. Chem. 282:26687.
  17. Razzaque, M.S. and B. Lanske (2007) J. Endocrinol. 194:1.
  18. Liu, S. et al. (2007) Curr. Opin. Nephrol. Hypertens. 16:329.

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Publications for FGF-23 (2629-FG/CF)(14)

We have publications tested in 4 confirmed species: Mouse, Rat, Canine, Mice.

We have publications tested in 2 applications: Bioassay, In Vivo.


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Bioassay
(10)
In Vivo
(4)
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Mouse
(10)
Rat
(1)
Canine
(1)
Mice
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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using 2629-FG/CF Applications Species
L Kägi, C Bettoni, EM Pastor-Arr, U Schnitzbau, N Hernando, CA Wagner Regulation of vitamin D metabolizing enzymes in murine renal and extrarenal tissues by dietary phosphate, FGF23, and 1,25(OH)2D3 PLoS ONE, 2018;13(5):e0195427. 2018 [PMID: 29771914] (In Vivo, Mice) In Vivo Mice
W Si, Y Zhang, K Chen, D Hu, Z Qian, S Gong, H Li, Y Hao, J Tao Fibroblast growth factor type 1 receptor stimulation of T-type Cachannels in sensory neurons requires the phosphatidylinositol 3-kinase and protein kinase A pathways, independently of Akt Cell. Signal., 2018;45(0):93-101. 2018 [PMID: 29382567] (Bioassay, Mouse) Bioassay Mouse
M Kuroda, R Muramatsu, N Maedera, Y Koyama, M Hamaguchi, H Fujimura, M Yoshida, M Konishi, N Itoh, H Mochizuki, T Yamashita Peripherally derived FGF21 promotes remyelination in the central nervous system J. Clin. Invest., 2017;0(0):. 2017 [PMID: 28825598] (Bioassay, Mouse) Bioassay Mouse
ER Smith, SJ Tan, SG Holt, TD Hewitson FGF23 is synthesised locally by renal tubules and activates injury-primed fibroblasts Sci Rep, 2017;7(1):3345. 2017 [PMID: 28611350] (Bioassay, Mouse) Bioassay Mouse
A Grabner, K Schramm, N Silswal, M Hendrix, C Yanucil, B Czaya, S Singh, M Wolf, S Hermann, J Stypmann, GS Di Marco, M Brand, MJ Wacker, C Faul FGF23/FGFR4-mediated left ventricular hypertrophy is reversible Sci Rep, 2017;7(1):1993. 2017 [PMID: 28512310] (Bioassay, Mouse) Bioassay Mouse
M Guibert, A Gasser, H Kempf, A Bianchi Fibroblast-growth factor 23 promotes terminal differentiation of ATDC5 cells PLoS ONE, 2017;12(4):e0174969. 2017 [PMID: 28406928] (Bioassay, Mouse) Bioassay Mouse
K Kawakami, A Takeshita, K Furushima, M Miyajima, I Hatamura, M Kuro-O, Y Furuta, K Sakaguchi Persistent fibroblast growth factor 23 signalling in the parathyroid glands for secondary hyperparathyroidism in mice with chronic kidney disease Sci Rep, 2017;7(0):40534. 2017 [PMID: 28094278] (Bioassay, Mouse) Bioassay Mouse
Lindberg I, Pang H, Stains J, Clark D, Yang A, Bonewald L, Li K FGF23 is endogenously phosphorylated in bone cells. J Bone Miner Res, 2015;30(3):449-54. 2015 [PMID: 25195776] (Bioassay) Bioassay
Ohata Y, Yamazaki M, Kawai M, Tsugawa N, Tachikawa K, Koinuma T, Miyagawa K, Kimoto A, Nakayama M, Namba N, Yamamoto H, Okano T, Ozono K, Michigami T Elevated fibroblast growth factor 23 exerts its effects on placenta and regulates vitamin D metabolism in pregnancy of Hyp mice. J Bone Miner Res, 2014;29(7):1627-38. 2014 [PMID: 24470103] (In Vivo, Mouse) In Vivo Mouse
Andrukhova O, Smorodchenko A, Egerbacher M, Streicher C, Zeitz U, Goetz R, Shalhoub V, Mohammadi M, Pohl E, Lanske B, Erben R FGF23 promotes renal calcium reabsorption through the TRPV5 channel. EMBO J, 2014;33(3):229-46. 2014 [PMID: 24434184] (Bioassay, Canine) Bioassay Canine
Show All 14 Publications.

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Bioinformatics

Gene Symbol Fgf23
Entrez
Uniprot