Recombinant Mouse FGF-23 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse FGF-23 Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human FGF RIIIc. The ED₅₀ for this effect is 0.2‑1.2 µg/mL in the presence of Recombinant Mouse Klotho (Catalog # 1819-KL) and heparin.
• Source: Mouse myeloma cell line, NS0-derived mouse FGF-23 protein
  Tyr25-Val251 (Arg179Gln), with a C-terminal 6-His tag
• Accession #: Q9EPC2
• N-terminal Sequence: Tyr25
• Protein/Peptide Type: Recombinant Proteins
• Gene: Fgf23
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 26.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 30-32 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in MOPS, Na₂SO₄, EDTA and DTT.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse FGF-23 Protein, CF

• ADHR
• FGF23
• FGF-23
• fibroblast growth factor 23
• HPDR2
• HYPF
• phosphatonin
• PHPTC
• tumor-derived hypophosphatemia inducing factor
• Tumor-derived hypophosphatemia-inducing factor

Background

Fibroblast growth factor 23 (FGF-23) is a 30 - 32 kDa member of the FGF gene family. Based on its structure, it is further classified as an FGF19 subfamily member. This subfamily includes FGF-19, -21, and -23. Like all other FGF subfamilies, FGF-19 subfamily members contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Unlike other FGF subfamilies, FGF-19 subfamily members exist as highly diffusible molecules that is attributed to poor ECM/heparin sulfate binding (3, 4, 5, 6). The cDNA for mouse FGF-23 predicts a 251 aa polypeptide that contains a 24 aa signal sequence and a 227 aa mature region (7). Mature mouse FGF-23 shows 72% aa identity to human FGF-23 (8). The FGF-19 subfamily shares an unusual receptor configuration. The standard model for FGF signaling requires an FGF:FGFR:heparin sulfate complex. Given FGF-23’s minimal association with heparin, a substitute termed ( alpha -) Klotho has evolved that serves the same function. Although FGF-23 binds to the widely expressed “c” isoforms of FGFR1 and 3 plus FGFR4, Klotho has a restricted distribution that limits FGF-23 activity (10, 11, 12). It should be noted that heparin-dependency has been reported for FGF-19 signaling, and this observation may extend to FGF-23 (13). The FGF-19 subfamily is considered endocrine in nature. All three subfamily members impact some aspect of metabolism and all three are induced by a nuclear receptor heterodimer that includes the retinoid X receptor (14, 15, 16). FGF-23 is considered a phosphatonin; that is, a molecule that reduces circulating plasma phosphate. It is produced by osteocytes and osteoblasts in response to high circulating phosphate levels, elevated parathyroid hormone that induces hypercalcemia, and circulatory volume loading. Upon binding to FGF-23 receptors on renal proximal tubular epithelium, two basic changes are seen. First, the enzyme responsible for generating the active form of vitamin D is suppressed, resulting in decreased levels of bioactive vitamin D. Since vitamin D promotes intestinal phosphate absorption, plasma phosphate declines. Second, the transporters responsible for phosphate resorption on renal epithelium are down regulated, resulting in decreased uptake from urine and again a decline in blood phosphorus (17, 18).

1. Itoh, N. and D.M. Ornitz (2004) Trends Genet. 20:563. 
2. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
3. Fukumoto, S. (2007) Endocr. J. Sep 14; [Epub ahead of print].
4. Huang, X. et al. (2006) Mol. Carcinog. 45:934. 
5. Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417.
6. Harmer, N.J. et al. (2004) Biochemistry 43:629.
7. Yamashita, T. et al. (2000) Biochem. Biophys. Res. Commun. 277:494.
8. Shimada, T. et al. (2001) Proc. Natl. Acad. Sci. USA 98:6500.
9. Kato, K. et al. (2006) J. Biol. Chem. 281:18370.
10. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
11. Urakawa, I. et al. (2006) Nature 444:770.
12. Hurosu, H. et al. (2006) J. Biol. Chem. 281:6120.
13. Wu, X. et al. (2007) J. Biol. Chem. 282:29069.
14. Moore, D.D. (2007) Science 316:1436.
15. Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci. USA 104:7432.
16. Kurosu, H. et al. (2007) J. Biol. Chem. 282:26687.
17. Razzaque, M.S. and B. Lanske (2007) J. Endocrinol. 194:1.
18. Liu, S. et al. (2007) Curr. Opin. Nephrol. Hypertens. 16:329.

Publications for FGF-23 (2629-FG/CF)(25)

Publications using 2629-FG/CF

• MB Meyer, NA Benkusky, SM Lee, SH Yoon, M Mannstadt, MN Wein, JW Pike Rapid genomic changes by mineralotropic hormones and kinase SIK inhibition drive coordinated renal Cyp27b1 and Cyp24a1 expression via CREB modules The Journal of Biological Chemistry, 2022-09-30;0(0):102559. 2022-09-30 [PMID: 36183832] (In Vivo, Mouse)
• B Czaya, K Heitman, I Campos, C Yanucil, D Kentrup, D Westbrook, O Gutierrez, JL Babitt, G Jung, IB Salusky, M Hanudel, C Faul Hyperphosphatemia increases inflammation to exacerbate anemia and skeletal muscle wasting independently of FGF23-FGFR4 signaling Elife, 2022-03-18;11(0):. 2022-03-18 [PMID: 35302487] (Cell Culture, Mouse)
• JA Navarro-Ga, R Salguero-B, L González-L, L Martín-Nun, E Rodríguez-, T Bada-Bosch, E Hernández, E Mérida-Her, M Praga, J Solís, F Arribas, H Bueno, M Kuro-O, M Fernández-, LM Ruilope, C Delgado, G Ruiz-Hurta The anti-aging factor Klotho protects against acquired long QT syndrome induced by uremia and promoted by fibroblast growth factor 23 Bmc Medicine, 2022-01-19;20(1):14. 2022-01-19 [PMID: 35042527] (In Vivo, Mouse)
• TW Lee, CC Chung, TI Lee, YK Lin, YH Kao, YJ Chen Fibroblast Growth Factor 23 Stimulates Cardiac Fibroblast Activity through Phospholipase C-Mediated Calcium Signaling International Journal of Molecular Sciences, 2021-12-23;23(1):. 2021-12-23 [PMID: 35008591] (Bioassay, Human)
• WY Chen Soluble Alpha-Klotho Alleviates Cardiac Fibrosis without Altering Cardiomyocytes Renewal Int J Mol Sci, 2020-03-22;21(6):. 2020-03-22 [PMID: 32235720] (Bioassay, Rat)
• S Westphal, T Gantert, C Kless, K Hüttinger, M Klingenspo, T Fromme Fibroblast growth factor 8b induces uncoupling protein 1 expression in epididymal white preadipocytes Sci Rep, 2019-06-11;9(1):8470. 2019-06-11 [PMID: 31186471] (Bioassay, Mouse)
• MB Meyer, NA Benkusky, M Kaufmann, SM Lee, RR Redfield, G Jones, JW Pike Targeted genomic deletions identify diverse enhancer functions and generate a kidney-specific, endocrine-deficient Cyp27b1 pseudo-null mouse J. Biol. Chem., 2019-05-03;0(0):. 2019-05-03 [PMID: 31053643] (In Vivo, Mouse)
• L Zhu, LR Stein, D Kim, K Ho, GQ Yu, L Zhan, TE Larsson, L Mucke Klotho controls the brain-immune system interface in the choroid plexus Proc. Natl. Acad. Sci. U.S.A., 2018-11-09;0(0):. 2018-11-09 [PMID: 30413620] (Bioassay, Mouse)
• N Quarto, S Shailendra, NP Meyer, S Menon, A Renda, MT Longaker Twist1-Haploinsufficiency Selectively Enhances the Osteoskeletal Capacity of Mesoderm-Derived Parietal Bone Through Downregulation of Fgf23 Front Physiol, 2018-10-15;9(0):1426. 2018-10-15 [PMID: 30374308] (Bioassay, Mouse)
• L Kägi, C Bettoni, EM Pastor-Arr, U Schnitzbau, N Hernando, CA Wagner Regulation of vitamin D metabolizing enzymes in murine renal and extrarenal tissues by dietary phosphate, FGF23, and 1,25(OH)2D3 PLoS ONE, 2018-05-17;13(5):e0195427. 2018-05-17 [PMID: 29771914] (In Vivo, Mouse)
• M Yashiro, M Ohya, T Mima, Y Ueda, Y Nakashima, K Kawakami, Y Ishizawa, S Yamamoto, S Kobayashi, T Yano, Y Tanaka, K Okuda, T Sonou, T Shoshihara, Y Iwashita, Y Iwashita, K Tatsuta, R Matoba, S Negi, T Shigematsu FGF23 modulates the effects of erythropoietin on gene expression in renal epithelial cells Int J Nephrol Renovasc Dis, 2018-04-04;11(0):125-136. 2018-04-04 [PMID: 29670389] (Bioassay, Mouse)
• R Agoro, A Montagna, R Goetz, O Aligbe, G Singh, LM Coe, M Mohammadi, S Rivella, D Sitara Inhibition of fibroblast growth factor 23 (FGF23) signaling rescues renal anemia FASEB J., 2018-02-26;0(0):fj201700667R. 2018-02-26 [PMID: 29481308] (In Vivo, Mouse)
• W Si, Y Zhang, K Chen, D Hu, Z Qian, S Gong, H Li, Y Hao, J Tao Fibroblast growth factor type 1 receptor stimulation of T-type Cachannels in sensory neurons requires the phosphatidylinositol 3-kinase and protein kinase A pathways, independently of Akt Cell. Signal., 2018-01-31;45(0):93-101. 2018-01-31 [PMID: 29382567] (Bioassay, Mouse)
• M Kuroda, R Muramatsu, N Maedera, Y Koyama, M Hamaguchi, H Fujimura, M Yoshida, M Konishi, N Itoh, H Mochizuki, T Yamashita Peripherally derived FGF21 promotes remyelination in the central nervous system J. Clin. Invest., 2017-08-21;0(0):. 2017-08-21 [PMID: 28825598] (Bioassay, Mouse)
• ER Smith, SJ Tan, SG Holt, TD Hewitson FGF23 is synthesised locally by renal tubules and activates injury-primed fibroblasts Sci Rep, 2017-06-13;7(1):3345. 2017-06-13 [PMID: 28611350] (Bioassay, Mouse)
• A Grabner, K Schramm, N Silswal, M Hendrix, C Yanucil, B Czaya, S Singh, M Wolf, S Hermann, J Stypmann, GS Di Marco, M Brand, MJ Wacker, C Faul FGF23/FGFR4-mediated left ventricular hypertrophy is reversible Sci Rep, 2017-05-16;7(1):1993. 2017-05-16 [PMID: 28512310] (Bioassay, Mouse, Rat)
• M Guibert, A Gasser, H Kempf, A Bianchi Fibroblast-growth factor 23 promotes terminal differentiation of ATDC5 cells PLoS ONE, 2017-04-13;12(4):e0174969. 2017-04-13 [PMID: 28406928] (Bioassay, Mouse)
• K Kawakami, A Takeshita, K Furushima, M Miyajima, I Hatamura, M Kuro-O, Y Furuta, K Sakaguchi Persistent fibroblast growth factor 23 signalling in the parathyroid glands for secondary hyperparathyroidism in mice with chronic kidney disease Sci Rep, 2017-01-17;7(0):40534. 2017-01-17 [PMID: 28094278] (Bioassay, Mouse)
• Lindberg I, Pang H, Stains J, Clark D, Yang A, Bonewald L, Li K FGF23 is endogenously phosphorylated in bone cells. J Bone Miner Res, 2015-03-01;30(3):449-54. 2015-03-01 [PMID: 25195776] (Bioassay)
• Ohata Y, Yamazaki M, Kawai M, Tsugawa N, Tachikawa K, Koinuma T, Miyagawa K, Kimoto A, Nakayama M, Namba N, Yamamoto H, Okano T, Ozono K, Michigami T Elevated fibroblast growth factor 23 exerts its effects on placenta and regulates vitamin D metabolism in pregnancy of Hyp mice. J Bone Miner Res, 2014-07-01;29(7):1627-38. 2014-07-01 [PMID: 24470103] (In Vivo, Mouse)
• Andrukhova O, Smorodchenko A, Egerbacher M, Streicher C, Zeitz U, Goetz R, Shalhoub V, Mohammadi M, Pohl E, Lanske B, Erben R FGF23 promotes renal calcium reabsorption through the TRPV5 channel. EMBO J, 2014-01-16;33(3):229-46. 2014-01-16 [PMID: 24434184] (Bioassay, Canine)
• Touchberry C, Green T, Tchikrizov V, Mannix J, Mao T, Carney B, Girgis M, Vincent R, Wetmore L, Dawn B, Bonewald L, Stubbs J, Wacker M FGF23 is a novel regulator of intracellular calcium and cardiac contractility in addition to cardiac hypertrophy. Am J Physiol Endocrinol Metab, 2013-02-26;304(8):E863-73. 2013-02-26 [PMID: 23443925] (Bioassay, Mouse)
• Lau , Wei Ling, Leaf , Elizabet, Hu , Ming Cha, Takeno , Marc M, Kuro-o , Makoto, Moe , Orson W, Giachelli , Cecilia Vitamin D receptor agonists increase klotho and osteopontin while decreasing aortic calcification in mice with chronic kidney disease fed a high phosphate diet. Kidney Int, 2012-08-29;82(12):1261-70. 2012-08-29 [PMID: 22932118] (Bioassay, Mouse)
• Faul C, Amaral AP, Oskouei B, Hu MC, Sloan A, Isakova T, Gutierrez OM, Aguillon-Prada R, Lincoln J, Hare JM, Mundel P, Morales A, Scialla J, Fischer M, Soliman EZ, Chen J, Go AS, Rosas SE, Nessel L, Townsend RR, Feldman HI, St John Sutton M, Ojo A, Gadegbeku C, Di Marco GS, Reuter S, Kentrup D, Tiemann K, Brand M, Hill JA, Moe OW, Kuro-o M, Kusek JW, Keane MG, Wolf M FGF23 induces left ventricular hypertrophy. J. Clin. Invest., 2011-10-10;121(11):4393-408. 2011-10-10 [PMID: 21985788] (Bioassay, In Vivo, Mouse)
• Galitzer H, Ben-Dov IZ, Silver J, Naveh-Many T Parathyroid cell resistance to fibroblast growth factor 23 in secondary hyperparathyroidism of chronic kidney disease. Kidney Int., 2009-12-16;77(3):211-8. 2009-12-16 [PMID: 20016468] (Bioassay, In Vivo, Rat)

Bioinformatics

• Gene Symbol: Fgf23
• Uniprot:
  • Q9EPC2()