Recombinant Mouse Dkk-2 Protein, CF

Images

 
Recombinant Mouse Wnt‑3a (Catalog # 1324-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Mouse Dkk-2 (Catalog #2435-DKB/CF) inhibits a constant dose ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Mouse Dkk-2 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Recombinant Mouse Wnt‑3a (Catalog # 1324-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells. Recombinant Mouse Dkk-2 (Catalog # 2435-DKB/CF) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN). The ED50 for this effect is 100-600 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Dkk-2 protein
Ser26-Ile259, with Leu35Pro substitution and a C-terminal 10-His tag
Accession #
N-terminal Sequence
Ser26
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
29-35 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Dkk-2 Protein, CF

  • dickkopf (Xenopus laevis) homolog 2
  • dickkopf 2
  • dickkopf homolog 2 (Xenopus laevis)
  • dickkopf related protein-2
  • dickkopf-2
  • dickkopf-related protein 2
  • Dkk2
  • Dkk-2
  • hDkk-2

Background

Dickkopf related protein 2 (Dkk-2) is a member of the Dickkopf family of secreted Wnt modulators (1-3). Dkk proteins contain a signal peptide and two conserved cysteine-rich domains that are separated by a linker region. The second cysteine-rich domain mediates Dkk-2 binding activities, and its interaction with LRP beta propellers has been mapped (2-4, 7). The 226 aa, ~35 kDa mature mouse Dkk-2 shares  99%, 96%, 96%, 96% and 94% aa identity with rat, human, dog, horse and cow Dkk-2, respectively, and can activate the canonical Wnt signaling pathway in Xenopus embryos (5). Dkk proteins modify Wnt engagement of a receptor complex composed of a Frizzled protein and a low-density lipoprotein receptor-related protein, either LRP5 or LRP6 (3). When LRP6 is over-expressed, direct high-affinity binding of Dkk-2 to LRP can enhance canonical Wnt signaling (6-8). However, when Dkk-2 and LRP6 form a ternary complex with Kremen2, Wnt signaling is inhibited due to internalization of Dkk-2/LRP6/Krm2 complexes (9, 10). Thus, depending on the cellular context, Dkk-2 can either activate or inhibit canonical Wnt signaling (3). In contrast, binding of Dkk-1 or Dkk-4 to LRP is consistently antagonistic (3). Dkk proteins are expressed in mesenchymal tissues and control epithelial transformations. Dkk-2 expression has been studied most in bone and eye, although it is expressed as early as periimplantation in mice (11). Mouse Dkk-1 or Dkk-2 deficiencies have opposite effects on bone homeostasis, despite down-regulating Wnt antagonism in both cases (12, 13). Dkk-2 expression is induced by Wnts in bone, and is thought to enhance bone density by promoting terminal differentiation of osteoblasts and mineral deposition (12). In contrast, Dkk-1 negatively regulates late osteoblast proliferation, which limits bone density (13). Dkk-2-deficient mice are blind, exhibiting faulty differentiation of corneal epithelium and ectopic blood vessels in the periocular mesenchyme (14, 15).

  1. Monaghan, A.P. et al. (1999) Mech. Dev. 87:45.
  2. Krupnik, V.E. et al. (1999) Gene 238:301.
  3. Niehrs, C. (2006) Oncogene 25:7469.
  4. Chen, L. et al. (2008) J. Biol. Chem. 283:23364.
  5. Wu, W. et al. (2000) Current Biol. 10:1611.
  6. Mao, B. et al. (2001) Nature 411:321.
  7. Li, L. et al. (2002) J. Biol. Chem. 277:5977.
  8. Brott, B. and S.Y. Sokol (2002) Mol. Cell. Biol. 22:6100.
  9. Mao, B. et al. (2002) Nature 417:664.
  10. Mao, B. and C. Niehrs (2003) Gene 302:179.
  11. Zhang, Y. et al. (2009) J. Reprod. Dev. 55:17.
  12. Li, X. et al. (2005) Nat. Genet. 37:945.
  13. van der Horst, G. et al. (2005) J. Bone Miner. Res. 20:1867.
  14. Mukhopadhyay, M. et al. (2006) Development 133:2149.
  15. Gage, P.J. et al. (2008) Dev. Biol. 317:310.

Customers Who Viewed This Item Also Viewed...

NBP2-37430
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready
NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC, IF
AF6646
Species: Hu
Applications: WB
AF1505
Species: Hu
Applications: WB, Flow, CyTOF-ready
1269-DK/CF
Species: Hu
NBP2-67286
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP1-33560
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, KO
645-WN
Species: Hu, Mu
NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC, IF
NBP2-67117
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NLS6792
Species: Hu, Mu, Po, Bt, Bv, Ca, Eq, Ha, Mk, Pm, Rb
Applications: IHC, IHC-P
1169-FR/CF
Species: Mu
1827-SF/CF
Species: Hu
7584-SF/CF
Species: Hu

Publications for Dkk-2 (2435-DKB/CF) (0)

There are no publications for Dkk-2 (2435-DKB/CF).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Dkk-2 (2435-DKB/CF) (0)

There are no reviews for Dkk-2 (2435-DKB/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Dkk-2 (2435-DKB/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse Dkk-2 Protein, CF and receive a gift card or discount.

Bioinformatics

Uniprot