Recombinant Mouse C1q R1/CD93 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse C1q R1/CD93 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to induce TNF-alpha secretion by THP‑1 human acute monocytic leukemia cells. The ED50 for this effect is 6-30 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse C1q R1/CD93 protein
Mouse C1q R1
(Ala23-Asn572)
Accession # O89103
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Ala23
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Cd93
Purity
>90%, by SDS-PAGE with silver staining
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
85.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
120-130 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE with silver staining
Reconstitution Instructions
Reconstitute at 400 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse C1q R1/CD93 Fc Chimera Protein, CF

  • AA4 Antigen
  • C1q R1
  • C1q receptor 1
  • C1q Rp
  • C1q/MBL/SPA receptor
  • C1qR(P)
  • C1qR1
  • C1qRp
  • CD93 antigenC1qR
  • CD93 molecule
  • CD93
  • CDw93C1QR1
  • Collectin Receptor
  • Complement component 1 q subcomponent receptor 1
  • complement component 1, q subcomponent, receptor 1
  • complement component C1q receptor
  • dJ737E23.1
  • ECSM3
  • Ly68
  • Matrix-remodeling-associated protein 4
  • matrix-remodelling associated 4
  • MXRA4

Background

C1q R1, also known as CD93, C1qRp, and AA4, is an approximately 125 kDa transmembrane glycoprotein that is involved in various aspects of inflammatory reactions (1). Mature mouse CD93 consists of a 550 amino acid (aa) extracellular domain (ECD) that contains one C‑type lectin domain, five tandem EGF‑like repeats, and a mucin‑like region. This is followed by a 21 aa transmembrane segment and a 51 aa cytoplasmic domain (2, 3). Within the ECD, mouse CD93 shares 66% and 87% aa sequence identity with human and rat CD93, respectively. C1qR1/CD93 should not be confused with 60 kDa Calreticulin which is also known as C1q R1 (5, 6). Unlike Calreticulin, and despite its name, C1q R1/CD93 does not appear to bind the complement protein C1q (4, 6). CD93 is expressed by vascular endothelial cells (6) and by a variety of hematopoietic cells (4‑10). Various sized fragments of soluble CD93 (50‑75 kDa) can be shed from monocytes, neutrophils, and vascular endothelial cells following inflammatory stimulation, leaving a truncated form of the molecule embedded in the cell membrane (12‑14). Cross‑linking of cell surface CD93 enhances phagocytosis by monocytes, and enhances the uptake of apoptotic cells in vivo (11, 16). Soluble CD93 promotes the differentiation of monocytes to macrophages, the phagocytosis of apoptotic cells, and inflammatory responsiveness to multiple TLR ligands (13, 15). It is required for plasma cell longevity in the bone marrow, resulting in an extended humoral response (10).
  1. Greenlee-Wacker, M.C. et al. (2012) Curr. Drug Targets 13:411.
  2. Petrenko, O. et al. (1999) Immunity 10:691.
  3. Norsworthy, P.J. et al. (1999) Mamm. Genome 10:789.
  4. Steinberger, P. et al. (2002) J. Leukoc. Biol. 71:133.
  5. Nepomuceno, R.R. and A.J. Tenner (1998) J. Immunol. 160:1929.
  6. McGreal, E.P. et al. (2002) J. Immunol. 168:5222.
  7. Lovik, G. et al. (2001) Scand. J. Immunol. 53:410.
  8. Danet, G.H. et al. (2002) Proc. Natl. Acad. Sci. USA 99:10441.
  9. Ikewaki, N. et al. (2010) J. Clin. Immunol. 30:723.
  10. Chevrier, S. et al. (2009) Proc. Natl. Acad. Sci. USA 106:3895.
  11. Norsworthy, P.J. et al. (2004) J. Immunol. 172:3406.
  12. Bohlson, S.S. et al. (2005) J. Immunol. 175:1239.
  13. Greenlee, M.C. et al. (2009) Inflamm. Res. 58:909.
  14. Greenlee-Wacker, M.C. et al. (2011) J. Immunol. 187:3353.
  15. Jeon, J.W. et al. (2010) J. Immunol. 185:4921.
  16. Nepomuceno, R.R. et al. (1999) J. Immunol. 162:3583.

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Bioinformatics

Gene Symbol Cd93
Entrez
Uniprot