Recombinant Human Wnt-7a Protein


There are currently no images for Wnt-7a (3008-WN).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human Wnt-7a Protein Summary

Details of Functionality
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 1-3 µg/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a.
Optimal dilutions should be determined by each laboratory for each application.
Chinese Hamster Ovary cell line, CHO-derived human Wnt-7a protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>70%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
35.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
40-65 kDa, reducing conditions
Read Publications using
3008-WN in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
>70%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Wnt-7a Protein

  • protein Wnt-7a
  • proto-oncogene Wnt7a protein
  • wingless-type MMTV integration site family, member 7A
  • Wnt7a
  • Wnt-7a


Human Wnt-7a is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1). Wnts bind to receptors of the Frizzled family in conjunction with low-density lipoprotein receptor-related proteins (LRPs). Downstream effects of Wnt signaling occur through activation of one of three different intracellular pathways: the canonical Wnt pathway, Wnt/Ca2+ pathway, and planar cell polarity. The highly transforming Wnts, including Wnt-1, -3, -3a, -7a and -8 activate the canonical pathway, which regulates beta ‑catenin-mediated gene expression (1, 2). Human Wnt-7a is a 48 kDa secreted glycoprotein containing 24 cysteine residues that is expressed by epithelial and epithelially-derived cells of the placenta, kidney, testis, uterus, fetal lung and fetal and adult brain (3, 4). Palmitate modification of a cysteine residue has been shown for Wnt-3a; this site is conserved on all Wnts and is Cys73 on Wnt7a (5). When modified, increased hydrophobicity and activity is expected. Human Wnt-7a shows 97% aa identity with mouse, rat and dog Wnt-7a and 92% aa identity with chicken Wnt-7a. During development, Wnt7a is expressed by the dorsal ectoderm and drives expression of homeodomain transcription factors that control effectors important in patterning and cell fates in adjacent mesenchyme (6 - 10). When Wnt-7a is deleted, mice show disruption of dorsalization and anterior/posterior patterning during limb development and abnormalities in the reproductive tract (6 - 10). Wnt-7a is frequently downregulated in leukemia and lung cancers, potentially affecting homeobox (HOX) gene expression, differentiation state and growth control (11, 12). Roles for Wnt-7a have also been shown during formation of neural synapses, response of the uterus to estrogen and inflammatory cartilage destruction (10, 13, 14).

  1. Logan, C. Y. and R. Nusse (2004) Annu. Rev. Cell Dev. Biol. 20:781.
  2. Wong, G. T. et al. (1994) Mol. Cell. Biol. 14:6278.
  4. Bui, T. D. et al. (1997) Gene 189:25.
  5. Willert, K. et al. (2003) Nature 423:448.
  6. Parr, B. A. and A.P. McMahon (1995) Nature 374:350.
  7. Cygan, J. A. et al. (1997) Development 124:5021.
  8. Parr, B.A. and A.P. McMahon (1998) Nature 395:707.
  9. Miller, C. and D. Sassoon (1998) Development 125:3201.
  10. Carta, L. and D. Sassoon (2004) Biol. Reprod. 71:444.
  11. Calvo, R. et al. (2000) Proc. Natl. Acad. Sci. USA 97:12776.
  12. Winn, R.A. et al. (2005) J. Biol Chem. 280:19625.
  13. Hall, A.C. et al. (2000) Cell 100:525.
  14. Hwang, S.-G. et al. (2004) J. Biol. Chem 279:26597.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu
Species: Mu
Applications: WB, IHC
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC, IF
Species: Hu, Mu
Species: Hu
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Species: Hu
Applications: WB, IHC
Species: Hu
Species: Hu
Applications: IHC, IHC-P
Species: Hu
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, IHC
Species: Hu
Applications: WB
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu
Applications: Bioactivity

Publications for Wnt-7a (3008-WN)(18)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Zebrafish.

We have publications tested in 2 applications: Bioassay, In Vivo.

Filter By Application
In Vivo
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using 3008-WN Applications Species
YX Wang, P Feige, CE Brun, B Hekmatneja, NA Dumont, JM Renaud, S Faulkes, DE Guindon, MA Rudnicki EGFR-Aurka Signaling Rescues Polarity and Regeneration Defects in Dystrophin-Deficient Muscle Stem Cells by Increasing Asymmetric Divisions Cell Stem Cell, 2019;0(0):. 2019 [PMID: 30713094] (Bioassay, Mouse) Bioassay Mouse
SH Lee, SM Shin, P Zhong, HT Kim, DI Kim, JM Kim, W Do Heo, DW Kim, CY Yeo, CH Kim, QS Liu Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes Nat Commun, 2018;9(1):3434. 2018 [PMID: 30143647] (Bioassay, Rat) Bioassay Rat
X Cheng, H Huang, X Luo, B Shi, J Li Wnt7a induces satellite cell expansion, myofiber hyperplasia and hypertrophy in rat craniofacial muscle Sci Rep, 2018;8(1):10613. 2018 [PMID: 30006540] (In Vivo, Rat) In Vivo Rat
S Condello, LE Sima, C Ivan, H Cardenas, GE Schiltz, RK Mishra, D Matei Tissue transglutaminase regulates interactions between ovarian cancer stem cells and the tumor niche Cancer Res., 2018;0(0):. 2018 [PMID: 29510995] (Bioassay, Human) Bioassay Human
X Cheng, L Song, M Lan, B Shi, J Li Morphological and molecular comparisons between tibialis anterior muscle and levator veli palatini muscle: A preliminary study on their augmentation potential Exp Ther Med, 2018;15(1):247-253. 2018 [PMID: 29375687] (In Vivo, Rat) In Vivo Rat
Cheng X, Song L, Lan M, Shi B, Li J Morphological and molecular comparisons between tibialis anterior muscle and levator veli palatini muscle: A preliminary study on their augmentation potential. Exp Ther Med, 0;15(1):247-253. 0 [PMID: 29375687] (In Vivo, Rat) In Vivo Rat
J Jang, Y Jung, S Chae, SI Chung, SM Kim, Y Yoon WNT/?-catenin pathway modulates the TNF-?-induced inflammatory response in bronchial epithelial cells Biochem. Biophys. Res. Commun, 2017;0(0):. 2017 [PMID: 28137581] (Bioassay, Human) Bioassay Human
J Jang, Y Jung, Y Kim, EH Jho, Y Yoon LPS-induced inflammatory response is suppressed by Wnt inhibitors, Dickkopf-1 and LGK974 Sci Rep, 2017;7(0):41612. 2017 [PMID: 28128299] (Bioassay, Human) Bioassay Human
Omar Farah Porcupine-dependent Wnt signaling controls stromal proliferation and endometrial gland maintenance through the action of distinct WNTs Dev. Biol, 2016;0(0):. 2016 [PMID: 27965056] (In Vivo, Mouse) In Vivo Mouse
Tsai H, Deng W, Lai W, Chiu W, Yang C, Tsai Y, Hwang S, Renshaw P Wnts enhance neurotrophin-induced neuronal differentiation in adult bone-marrow-derived mesenchymal stem cells via canonical and noncanonical signaling pathways. PLoS ONE, 2014;9(8):e104937. 2014 [PMID: 25170755] (Bioassay, Human) Bioassay Human
Show All 18 Publications.

Reviews for Wnt-7a (3008-WN) (0)

There are no reviews for Wnt-7a (3008-WN). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Wnt-7a (3008-WN) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Human Wnt-7a Protein and receive a gift card or discount.


Gene Symbol WNT7A