Recombinant Human Wnt-7a Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human Wnt-7a Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 1-3 µg/mL in the presence of 5 ng/mL rmWnt-3a.
Optimal dilutions should be determined by each laboratory for each application.
Source
Chinese Hamster Ovary cell line, CHO-derived human Wnt-7a protein
Leu32-Lys349
Accession #
N-terminal Sequence
Leu32
Protein/Peptide Type
Recombinant Proteins
Gene
WNT7A
Purity
>70%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
35.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
40-65 kDa, reducing conditions
Publications
Read Publications using
3008-WN/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
Purity
>70%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Wnt-7a Protein, CF

  • protein Wnt-7a
  • proto-oncogene Wnt7a protein
  • wingless-type MMTV integration site family, member 7A
  • Wnt7a
  • Wnt-7a

Background

Human Wnt-7a is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1). Wnts bind to receptors of the Frizzled family in conjunction with low-density lipoprotein receptor-related proteins (LRPs). Downstream effects of Wnt signaling occur through activation of one of three different intracellular pathways: the canonical Wnt pathway, Wnt/Ca2+ pathway, and planar cell polarity. The highly transforming Wnts, including Wnt-1, -3, -3a, -7a and -8 activate the canonical pathway, which regulates beta -catenin-mediated gene expression (1, 2). Human Wnt-7a is a 48 kDa secreted glycoprotein containing 24 cysteine residues that is expressed by epithelial and epithelially-derived cells of the placenta, kidney, testis, uterus, fetal lung and fetal and adult brain (3, 4). Palmitate modification of a cysteine residue has been shown for Wnt-3a (5); this site is conserved on all Wnts and is Cys73 on Wnt7a. When modified, increased hydrophobicity and activity is expected. Human Wnt-7a shows 97% aa identity with mouse, rat and dog Wnt-7a and 92% aa identity with chicken Wnt-7a. During development, Wnt7a is expressed by the dorsal ectoderm and drives expression of homeodomain transcription factors that control effectors important in patterning and cell fates in adjacent mesenchyme (6 - 10). When Wnt-7a is deleted, mice show disruption of dorsalization and anterior/posterior patterning during limb development and abnormalities in the reproductive tract (6 - 10). Wnt-7a is frequently downregulated in leukemia and lung cancers, potentially affecting homeobox (HOX) gene expression, differentiation state and growth control (11, 12). Roles for Wnt-7a have also been shown during formation of neural synapses (13), response of the uterus to estrogen (10) and inflammatory cartilage destruction (14).

  1. Logan, C. Y. and R. Nusse (2004) Annu. Rev. Cell Dev. Biol. 20:781.
  2. Wong, G. T. et al. (1994) Mol. Cell. Biol. 14:6278.
  3. http://www.stanford.edu/~rnusse/wntwindow.html 
  4. Bui, T. D. et al. (1997) Gene 189:25.
  5. Willert, K. et al. (2003) Nature 423:448.. 
  6. Parr, B. A. and A.P. McMahon (1995) Nature 374:350.
  7. Cygan, J. A. et al. (1997) Development 124:5021.
  8. Parr, B.A. and A.P. McMahon (1998) Nature 395:707.
  9. Miller, C. and D. Sassoon (1998) Development 125:3201.
  10. Carta, L. and D. Sassoon (2004) Biol. Reprod. 71:444.
  11. Calvo, R. et al. (2000) Proc. Natl. Acad. Sci. USA 97:12776.
  12. Winn, R.A. et al. (2005) J. Biol Chem. 280:19625.
  13. Hall, A.C. et al. (2000) Cell 100:525.
  14. Hwang, S.-G. et al. (2004) J. Biol. Chem 279:26597.

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Publications for Wnt-7a (3008-WN/CF)(13)

We have publications tested in 3 confirmed species: Human, Mouse, Zebrafish.

We have publications tested in 2 applications: Bioassay, In Vivo.


Filter By Application
Bioassay
(10)
In Vivo
(2)
All Applications
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Human
(6)
Mouse
(5)
Zebrafish
(1)
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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using 3008-WN/CF Applications Species
S Condello, LE Sima, C Ivan, H Cardenas, GE Schiltz, RK Mishra, D Matei Tissue transglutaminase regulates interactions between ovarian cancer stem cells and the tumor niche Cancer Res., 2018;0(0):. 2018 [PMID: 29510995] (Bioassay, Human) Bioassay Human
J Jang, Y Jung, S Chae, SI Chung, SM Kim, Y Yoon WNT/?-catenin pathway modulates the TNF-?-induced inflammatory response in bronchial epithelial cells Biochem. Biophys. Res. Commun, 2017;0(0):. 2017 [PMID: 28137581] (Bioassay, Human) Bioassay Human
J Jang, Y Jung, Y Kim, EH Jho, Y Yoon LPS-induced inflammatory response is suppressed by Wnt inhibitors, Dickkopf-1 and LGK974 Sci Rep, 2017;7(0):41612. 2017 [PMID: 28128299] (Bioassay, Human) Bioassay Human
Omar Farah Porcupine-dependent Wnt signaling controls stromal proliferation and endometrial gland maintenance through the action of distinct WNTs Dev. Biol, 2016;0(0):. 2016 [PMID: 27965056] (In Vivo, Mouse) In Vivo Mouse
Tsai H, Deng W, Lai W, Chiu W, Yang C, Tsai Y, Hwang S, Renshaw P Wnts enhance neurotrophin-induced neuronal differentiation in adult bone-marrow-derived mesenchymal stem cells via canonical and noncanonical signaling pathways. PLoS ONE, 2014;9(8):e104937. 2014 [PMID: 25170755] (Bioassay, Human) Bioassay Human
Kilander M, Dahlstrom J, Schulte G Assessment of Frizzled 6 membrane mobility by FRAP supports G protein coupling and reveals WNT-Frizzled selectivity. Cell Signal, 2014;26(9):1943-9. 2014 [PMID: 24873871] (Bioassay, Human) Bioassay Human
Bentzinger C, von Maltzahn J, Dumont N, Stark D, Wang Y, Nhan K, Frenette J, Cornelison D, Rudnicki M Wnt7a stimulates myogenic stem cell motility and engraftment resulting in improved muscle strength. J Cell Biol, 2014;205(1):97-111. 2014 [PMID: 24711502]
Okamoto M, Udagawa N, Uehara S, Maeda K, Yamashita T, Nakamichi Y, Kato H, Saito N, Minami Y, Takahashi N, Kobayashi Y Noncanonical Wnt5a enhances Wnt/beta-catenin signaling during osteoblastogenesis. Sci Rep, 2014;4(0):4493. 2014 [PMID: 24670389] (Bioassay, Mouse) Bioassay Mouse
Thrasivoulou C, Millar M, Ahmed A Activation of intracellular calcium by multiple Wnt ligands and translocation of beta-catenin into the nucleus: a convergent model of Wnt/Ca2+ and Wnt/beta-catenin pathways. J Biol Chem, 2013;288(50):35651-9. 2013 [PMID: 24158438] (Bioassay, Human) Bioassay Human
Wnt7a treatment ameliorates muscular dystrophy Proc. Natl. Acad. Sci. U.S.A., 2012;109(0):20614-20619. 2012 [PMID: 23185011] (Bioassay, Mouse) Bioassay Mouse
Show All 13 Publications.

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Bioinformatics

Gene Symbol WNT7A
Entrez
Uniprot