Recombinant Human Wnt-7a Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications BA
Format
Carrier-Free

Order Details

Recombinant Human Wnt-7a Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 1-3 µg/mL in the presence of 5 ng/mL rmWnt-3a.
Optimal dilutions should be determined by each laboratory for each application.
Source
Chinese Hamster Ovary cell line, CHO-derived
Leu32-Lys349
Accession #
N-terminal Sequence
Leu32
Protein/Peptide Type
Recombinant Proteins
Gene
WNT7A
Purity
>70%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
35.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
40-65 kDa, reducing conditions
Publications
Read Publications using
3008-WN/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
Purity
>70%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Wnt-7a Protein, CF

  • protein Wnt-7a
  • proto-oncogene Wnt7a protein
  • wingless-type MMTV integration site family, member 7A
  • Wnt7a
  • Wnt-7a

Background

Human Wnt-7a is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1). Wnts bind to receptors of the Frizzled family in conjunction with low-density lipoprotein receptor-related proteins (LRPs). Downstream effects of Wnt signaling occur through activation of one of three different intracellular pathways: the canonical Wnt pathway, Wnt/Ca2+ pathway, and planar cell polarity. The highly transforming Wnts, including Wnt-1, -3, -3a, -7a and -8 activate the canonical pathway, which regulates beta -catenin-mediated gene expression (1, 2). Human Wnt-7a is a 48 kDa secreted glycoprotein containing 24 cysteine residues that is expressed by epithelial and epithelially-derived cells of the placenta, kidney, testis, uterus, fetal lung and fetal and adult brain (3, 4). Palmitate modification of a cysteine residue has been shown for Wnt-3a (5); this site is conserved on all Wnts and is Cys73 on Wnt7a. When modified, increased hydrophobicity and activity is expected. Human Wnt-7a shows 97% aa identity with mouse, rat and dog Wnt-7a and 92% aa identity with chicken Wnt-7a. During development, Wnt7a is expressed by the dorsal ectoderm and drives expression of homeodomain transcription factors that control effectors important in patterning and cell fates in adjacent mesenchyme (6 - 10). When Wnt-7a is deleted, mice show disruption of dorsalization and anterior/posterior patterning during limb development and abnormalities in the reproductive tract (6 - 10). Wnt-7a is frequently downregulated in leukemia and lung cancers, potentially affecting homeobox (HOX) gene expression, differentiation state and growth control (11, 12). Roles for Wnt-7a have also been shown during formation of neural synapses (13), response of the uterus to estrogen (10) and inflammatory cartilage destruction (14).

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Publications for (10)

We have publications tested in 3 confirmed species: Human, Mouse, Zebrafish.

We have publications tested in 2 applications: Bioassay, In Vivo.


Filter By Application
Bioassay
(8)
In Vivo
(1)
All Applications
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Human
(4)
Mouse
(4)
Zebrafish
(1)
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Showing Publications 1 - 10 of 10.
Publications using 3008-WN/CF Applications Species
J Jang, Y Jung, Y Kim, EH Jho, Y Yoon LPS-induced inflammatory response is suppressed by Wnt inhibitors, Dickkopf-1 and LGK974 Sci Rep, 2017;7(0):41612. 2017 [PMID: 28128299] (Bioassay, Human) Bioassay Human
Tsai H, Deng W, Lai W, Chiu W, Yang C, Tsai Y, Hwang S, Renshaw P Wnts enhance neurotrophin-induced neuronal differentiation in adult bone-marrow-derived mesenchymal stem cells via canonical and noncanonical signaling pathways. PLoS ONE, 2014;9(8):e104937. 2014 [PMID: 25170755] (Bioassay, Human) Bioassay Human
Kilander M, Dahlstrom J, Schulte G Assessment of Frizzled 6 membrane mobility by FRAP supports G protein coupling and reveals WNT-Frizzled selectivity. Cell Signal, 2014;26(9):1943-9. 2014 [PMID: 24873871] (Bioassay, Human) Bioassay Human
Bentzinger C, von Maltzahn J, Dumont N, Stark D, Wang Y, Nhan K, Frenette J, Cornelison D, Rudnicki M Wnt7a stimulates myogenic stem cell motility and engraftment resulting in improved muscle strength. J Cell Biol, 2014;205(1):97-111. 2014 [PMID: 24711502]
Okamoto M, Udagawa N, Uehara S, Maeda K, Yamashita T, Nakamichi Y, Kato H, Saito N, Minami Y, Takahashi N, Kobayashi Y Noncanonical Wnt5a enhances Wnt/beta-catenin signaling during osteoblastogenesis. Sci Rep, 2014;4(0):4493. 2014 [PMID: 24670389] (Bioassay, Mouse) Bioassay Mouse
Thrasivoulou C, Millar M, Ahmed A Activation of intracellular calcium by multiple Wnt ligands and translocation of beta-catenin into the nucleus: a convergent model of Wnt/Ca2+ and Wnt/beta-catenin pathways. J Biol Chem, 2013;288(50):35651-9. 2013 [PMID: 24158438] (Bioassay, Human) Bioassay Human
Wnt7a treatment ameliorates muscular dystrophy Proc. Natl. Acad. Sci. U.S.A., 2012;109(0):20614-20619. 2012 [PMID: 23185011] (Bioassay, Mouse) Bioassay Mouse
Tran, Thanh H, Shi, Xiaofeng, Zaia, Joseph, Ai, Xingbin Heparan sulfate 6-O-endosulfatases (Sulfs) coordinate the Wnt signaling pathways to regulate myoblast fusion during skeletal muscle regeneration. J Biol Chem, 2012;287(39):32651-64. 2012 [PMID: 22865881] (Bioassay, Mouse) Bioassay Mouse
Gogolla N, Galimberti I, Deguchi Y, Caroni P Wnt signaling mediates experience-related regulation of synapse numbers and mossy fiber connectivities in the adult hippocampus. Neuron, 2009;62(4):510-25. 2009 [PMID: 19477153] (In Vivo, Mouse) In Vivo Mouse
Ma LH, Webb SE, Chan CM, Zhang J, Miller AL Establishment of a transitory dorsal-biased window of localized Ca2+ signaling in the superficial epithelium following the mid-blastula transition in zebrafish embryos. Dev. Biol., 2009;327(1):143-57. 2009 [PMID: 19133253] (Bioassay, Zebrafish) Bioassay Zebrafish

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Bioinformatics

Gene Symbol WNT7A
Entrez
Uniprot